Compounds, compositions, and methods

ABSTRACT

Compounds, compositions and methods useful for treating cellular proliferative diseases and disorders, for example, by modulating the activity of KSP, are disclosed.

CROSS-REFERENCE TO RELATED APPLICATIONS

This application claims the benefit of co-pending provisional U.S.Application Ser. No. 60/414,756, filed Sep. 30, 2002 incorporated hereinby reference.

FIELD OF THE INVENTION

This invention relates to quinazolinone-like derivatives that areinhibitors of the mitotic kinesin KSP and are useful in the treatment ofcellular proliferative diseases, for example cancer, hyperplasias,restenosis, cardiac hypertrophy, immune disorders and inflammation.

BACKGROUND OF THE INVENTION

The mitotic spindle is responsible for distribution of replicate copiesof the genome to each of the two daughter cells that result from celldivision. Disruption of the mitotic spindle can inhibit cell division,and induce cell death. Microtubules are the primary structural elementof the mitotic spindle; they are the site of action of certain existingtherapeutic agents used to treat cancer, such as taxanes and vincaalkaloids. Microtubules, however, exist as elements in other types ofcellular structures (including tracks for intracellular transport innerve processes). The therapeutic targeting of microtubules can,therefore, modulate processes in addition to cellular proliferation,leading to side effects that limit the usefulness of such drugs.

Improvement in the specificity of agents used to treat cancer is ofconsiderable interest because of the therapeutic benefits that would berealized if the side effects associated with the administration of theseagents could be reduced. Dramatic improvements in the treatment ofcancer have been associated with identification of therapeutic agentsacting through novel mechanisms. Examples of this include not only thetaxanes, but also the camptothecin class of topoisomerase I inhibitors.

One novel anti-proliferative mechanism entails selective inhibition ofmitotic kinesins, enzymes that are essential for assembly and functionof the mitotic spindle, but are not generally part of other microtubulestructures, such as in nerve processes. See, e.g., Guidebook to theCytoskeletal and Motor Proteins, Kreis and Vale, Eds., pp. 389-394(Oxford University Press 1999). Mitotic kinesins play essential rolesduring all phases of mitosis. These enzymes are “molecular motors” thattransform energy released by hydrolysis of ATP into mechanical forcethat drives the directional movement of cellular cargoes alongmicrotubules. The catalytic domain sufficient for this task is a compactstructure of approximately 340 amino acids. During mitosis, kinesinsorganize microtubules into the bipolar structure that is the mitoticspindle. Kinesins mediate movement of chromosomes along spindlemicrotubules, as well as structural changes in the mitotic spindleassociated with specific phases of mitosis. Experimental perturbation ofmitotic kinesin function causes malformation or dysfunction of themitotic spindle, frequently resulting in cell cycle arrest and celldeath. Mitotic kinesins are attractive targets for the discovery anddevelopment of novel anti-mitotic chemotherapeutics.

Among the mitotic kinesins that have been identified is KSP. KSP belongsto an evolutionarily conserved kinesin subfamily of plus end-directedmicrotubule motors that assemble into bipolar homotetramers consistingof antiparallel homodimers. During mitosis, KSP associates withmicrotubules of the mitotic spindle. Microinjection of antibodiesdirected against KSP into human cells prevents spindle pole separationduring prometaphase, giving rise to monopolar spindles and causingmitotic arrest and induction of programmed cell death. KSP and relatedkinesins in other, non-human, organisms, bundle antiparallelmicrotubules and slide them relative to one another, thus forcing thetwo spindle poles apart. KSP may also mediate in anaphase B spindleelongation and focussing of microtubules at the spindle pole.

Human KSP (also termed HsEg5) has been described [Blangy, et al., Cell,83:1159-69 (1995); Whitehead, et al., Arthritis Rheum., 39:1635-42(1996); Galgio et al., J. Cell Biol., 135:339-414 (1996); Blangy, etal., J Biol. Chem., 272:19418-24 (1997); Blangy, et al., Cell Motil.Cytoskeleton, 40:174-82 (1998); Whitehead and Rattner, J. Cell Sci.,111:2551-61 (1998); Kaiser, et al., JBC 274:18925-31 (1999); GenBankaccession numbers: X85137, NM004523 and U37426], and a fragment of theKSP gene (TRIP5) has been described [Lee, et al., Mol. Endocrinol.,9:243-54 (1995); GenBank accession number L40372]. Xenopus KSP homologs(Eg5), as well as Drosophila KLP61 F/KRP1 30 have been reported.

Recently, certain substituted quinazolinones have been described asinhibitors of mitotic kinesins for the treatment of cellularproliferative diseases (WO 01/30768 and WO 01/98278). It is an object ofthe present invention to provide novel inhibitors of mitotic kinesinssuch as KSP (particularly human KSP).

SUMMARY OF THE INVENTION

The present invention provides compounds, compositions and methodsuseful in the inhibition of mitotic kinesins, particularly KSP (moreparticularly human KSP). The compounds can be used to treat cellularproliferative diseases and include certain pyrrolidine- andpiperidine-substituted quinazolinone derivatives.

In one aspect, the invention relates to one or more compounds selectedfrom the group represented by Formula I:

where:

-   -   U—V is chosen from —N(R⁶)—CR^(e)R^(f)—, —CR^(e)R^(f)—N(R⁶)—,        —N(R⁶)CR^(e)R^(f)—CR^(g)R^(h)—, —CR^(e)R^(f)—N(R⁶)—CR^(g)R^(h),        and —CR^(e)R^(f)—CR^(g)R^(h)—N(R⁶)—;    -   R^(a), R^(b), R^(c), R^(d), R^(e), R^(f), R^(g) and R^(h) are        independently chosen from hydrogen, alkyl, aryl, aralkyl,        heteroaryl, substituted alkyl, substituted aryl, substituted        aralkyl, and substituted heteroaryl;    -   R¹, R², R³, and R⁴ are independently chosen from hydrogen,        alkyl, alkoxy, halogen, cyano and substituted alkyl;    -   R⁵ is chosen from alkyl, aryl, aralkyl, heteroaryl,        heteroaralkyl, substituted alkyl, substituted aryl, substituted        aralkyl, substituted heteroaryl and substituted heteroaralkyl;        and    -   R⁶ is chosen from hydrogen, acyl, alkyl, aryl, aralkyl,        heteroaryl, substituted acyl,

substituted alkyl, substituted aryl, substituted aralkyl and substitutedheteroaryl; including single stereoisomers, mixtures of stereoisomers,and pharmaceutically acceptable salts, solvates, and solvates ofpharmaceutically acceptable salts thereof. Compounds of Formula I andpharmaceutically acceptable salts and solvates thereof are useful asactive agents in the practice of the methods of treatment and inmanufacture of compositions including the pharmaceutical formulations ofthe invention, and may also be useful as intermediates in the synthesisof such active agents.

In another aspect, the invention relates to one or more compoundsselected from the group represented by Formula II:

where:

-   -   T is a covalent bond or optionally substituted lower alkylene;    -   W, X and Y are independently chosen from —N═, N, —C═, CH,        CR^(i), O and S;    -   Z is chosen from —N═, N, —C═, CH, CR^(i) or is absent, provided        that:        -   no more than two of W, X, Y and Z are —N═, and        -   W, X or Y can be O or S only when Z is absent;    -   R¹ is chosen from alkyl, alkoxy, halogen, cyano and substituted        alkyl; and    -   R¹ to R⁶, U and V are as defined with regard to Formula I,        provided that R¹, R², R³ or R⁴ is absent where W, X, Y or Z,        respectively, is —N═, O, S or absent;        including single stereoisomers, mixtures of stereoisomers, and        pharmaceutically acceptable salts, solvates, and solvates of        pharmaceutically acceptable salts thereof. The compounds        encompassed by Formula II and pharmaceutically acceptable salts        and solvates thereof will be seen to include those of Formula I;        they are likewise useful as active agents in the practice of the        methods of treatment and in manufacture of compositions        including the pharmaceutical formulations of the invention, and        may also be useful as intermediates in the synthesis of such        active agents. The dashed lines in Formula II indicate that the        corresponding bond may be a single bond (e.g., where X is CH) or        a double bond (e.g., where X is —C═).

In one of its particular aspects the present invention pertains to acompound represented by Formula I or II, having a substituent selectedfrom one or more of the following for R^(a) to R^(h), R¹ to R⁶, T, U, V,or W, X, Y and Z:

-   -   U—V is —N(R⁶)CR^(e)R^(f)—CR^(g)R^(h);    -   R^(a) to R^(h) are chosen from hydrogen, lower alkyl        (particularly methyl) and substituted lower alkyl;    -   R¹, R², R³ and R⁴ are independently chosen from hydrogen, halo        (particularly chloro and fluoro), lower alkyl (particularly        methyl), substituted lower alkyl, lower alkoxy (particularly        methoxy), and cyano;    -   R⁵ is aralkyl or substituted aralkyl (particularly benzyl or        substituted benzyl; most particularly benzyl);    -   R⁶ is acyl (particularly benzoyl), aryl (particularly phenyl),        substituted aryl (particularly lower alkyl-, lower alkoxy-,        and/or halo-substituted phenyl), aralkyl (particularly benzyl        and phenylviny), heteroaralkyl, oxaaralkyl (particularly phenoxy        lower alkyl), oxaheteroaralkyl, substituted acyl (particularly        p-toluoyl), substituted aralkyl (particularly substituted benzyl        and substituted phenylviny), substituted heteroaralkyl,        substituted oxaaralkyl (particularly substituted phenoxy lower        alkyl), or substituted oxaheteroaralkyl.    -   T is lower alkylene, substituted lower alkylene or a covalent        bond (particularly a covalent bond); and    -   W, X, Y and Z are C═ or —N═(particularly C═).        Other particular aspects of the invention pertain to methods and        to pharmaceutical formulations employing such a compound.

In one aspect, the invention relates to methods for treating cellularproliferative diseases, for disorders that can be treated by modulatingKSP kinesin activity, and for inhibiting KSP kinesin by theadministration of a therapeutically effective amount of a compound ofFormula I or II, or a pharmaceutically acceptable salt or solvate ofsuch compounds. Diseases and disorders that respond to therapy withcompounds of the invention include cancer, hyperplasia, restenosis,cardiac hypertrophy, immune disorders and inflammation.

In another aspect, the invention relates to a pharmaceutical compositioncontaining a therapeutically effective amount of a compound of Formula Ior II or a pharmaceutically acceptable salt or solvate thereof admixedwith at least one pharmaceutically acceptable excipient.

Yet another aspect of the invention relates to a kit having a compound,pharmaceutically acceptable salt or solvate of Formula I or II and apackage insert or other labeling including directions for treating acellular proliferative disease by administering an effective amount ofthe compound, salt or solvate. In one particular such aspect, thecompound, pharmaceutically acceptable salt or solvate of Formula I or IIis provided as a pharmaceutical composition.

In an additional aspect, the present invention provides methods ofscreening for compounds that will bind to a KSP kinesin, for examplecompounds that will displace or compete with the binding of thecompounds of the invention. The methods entail combining a labeledcompound of the invention, a KSP kinesin, and at least one candidateagent and determining the binding of the candidate agent to the KSPkinesin.

In a further aspect, the invention provides methods of screening formodulators of KSP kinesin activity. The methods entail combining acompound of the invention, a KSP kinesin, and at least one candidateagent and determining the effect of the candidate agent on the KSPkinesin activity.

DETAILED DESCRIPTION OF THE INVENTION

The present invention provides compounds, compositions and methodsuseful in the inhibition of mitotic kinesins, particularly KSP (moreparticularly human KSP). The compounds can be used to treat cellularproliferative diseases and include certain pyrrolidine- andpiperidine-substituted quinazolinone derivatives. The invention furtherrelates to pharmaceutical formulations comprising compounds of theinvention, and methods of treatment employing such compounds orcompositions.

Definitions

As used in the present specification, the following words and phrasesare generally intended to have the meanings as set forth below, exceptto the extent that the context in which they are used indicatesotherwise. The following abbreviations and terms have the indicatedmeanings throughout:

Ac=acetyl

Boc=t-butyloxy carbonyl

c-=cyclo

CBZ=carbobenzoxy=benzyloxycarbonyl

DCM=dichloromethane=methylene chloride=CH₂Cl₂

DMF=N,N-dimethylformamide

DMSO=dimethyl sulfoxide

Et=ethyl

PyBroP=bromo-tris-pyrrolidinophosphonium hexafluorophosphate

s-=secondary

t-=tertiary

TFA=trifluoroacetic acid

The substituents identified as U, V, W and Y are intended to have themeanings set forth in the Summary, this Detailed Description and theClaims; they are not intended to designate the atomic elements Uranium,Vanadium, Tungsten and Yttrium.

The term “optional” or “optionally” means that the subsequentlydescribed event or circumstance may or may not occur, and that thedescription includes instances where said event or circumstance occursand instances in which it does not. For example, “optionally substitutedalkyl” includes “alkyl” and “substituted alkyl,” as defined below. Itwill be understood by those skilled in the art with respect to any groupcontaining one or more substituents that such groups are not intended tointroduce any substitution or substitution patterns that are stericallyimpractical, synthetically non-feasible and/or inherently unstable.

“Alkyl” is intended to include linear, branched, or cyclic aliphatichydrocarbon structures and combinations thereof, which structures may besaturated or unsaturated (particularly having up to 20 carbon atoms,more particularly up to C₁₃.). Lower alkyl refers to alkyl groups offrom 1 to 5 (particularly 1 to 4) carbon atoms. Examples of lower alkylgroups include methyl, ethyl, propyl, isopropyl, butyl, s-and t-butyland the like. Cycloalkyl (or carbocyclic) is a subset of alkyl andincludes cyclic aliphatic hydrocarbon groups of from 3 to 13 carbonatoms. Examples of cycloalkyl groups include c-propyl, c-butyl,c-pentyl, norbornyl, adamantyl and the like. In this application, alkylrefers to alkanyl, alkenyl and alkynyl residues; it is intended toinclude cyclohexylmethyl, vinyl, allyl, isoprenyl and the like.Alkylene, alkenylene and alkynylene are other subsets of alkyl,referring to the same residues as alkyld but having two points ofattachment. Examples of alkylene include ethylene (—CH₂CH₂—), ethenylene(—CH═CH—), propylene (—CH₂CH₂CH₂—), dimethylpropylene (—CH₂C(CH₃)₂CH₂—)and cyclohexylpropylene (—CH₂CH₂CH(C₆H₁₃)—). When an alkyl residuehaving a specific number of carbons is named, all geometric isomers ofthat residue having the specified number of carbons are meant to beincluded; thus, for example, “butyl” is meant to include n-butyl,sec-butyl, isobutyl and t-butyl; “propyl” includes n-propyl andisopropyl.

The term “alkoxy” or “alkoxyl” refers to the group —O-alkyl,particularly including from 1 to 8 carbon atoms in a straight, branchedor cyclic configuration, or combinations thereof, attached to the parentstructure through an oxygen. Examples include methoxy, ethoxy, propoxy,isopropoxy, cyclopropyloxy, cyclohexyloxy and the like. Lower-alkoxyrefers to groups containing one to five carbons.

The term “substituted alkoxy” refers to the group —O-(substitutedalkyl). One particular substituted alkoxy group is “polyalkoxy” or—O-(optionally substituted alkylene)-(optionally substituted alkoxy),and includes groups such as —OCH₂CH₂OCH₃, and glycol ethers such aspolyethyleneglycol and —O(CH₂CH₂O)_(x)CH₃, where x is an integer ofabout 2-20, particularly about 2-10, and more particularly about 2-5.Another particular substituted alkoxy group is hydroxyalkoxy orOCH₂(CH₂)_(y)OH, where y is an integer of about 1-10, particularly about1-4.

“Acyl” refers to groups of from 1 to 8 carbon atoms in a straight,branched or cyclic configuration, or combinations thereof, or to ahydrogen atom attached to the parent structure through a carbonylfunctionality. Such groups may be saturated or unsaturated, andaliphatic or aromatic. One or more carbons in the acyl residue may bereplaced by nitrogen, oxygen or sulfur as long as the point ofattachment to the parrent remains at the carbonyl. Examples includeformyl, acetyl, benzoyl, propionyl, isobutyryl, t-butoxycarbonyl,benzyloxycarbonyl, aminocarbonyl, and the like. Lower-acyl refers to anacyl group containing one to five carbons. “Substituted acyl” refers toan acyl group where one or more of the hydrogens otherwise attached to acarbon, nitrogen or sulfur atom is substituted, the point of attachmentto the parent moiety remaining at the carbonyl.

The term “acyloxy” refers to the group —O-acyl. “Substituted acyloxy”refers to the group —O-substituted acyl.

The term “amidino” refers to the group C(═NH)—NH₂. The term “substitutedamidino” refers to the formula —C(═NR)—NRR in which each R isindependently selected from the group:

hydrogen, optionally substituted alkyl, optionally substituted alkoxy,optionally substituted aminocarbonyl, optionally substituted aryl,optionally substituted heteroaryl, optionally substituted heterocyclyl,acyl, alkoxycarbonyl, sulfanyl, sulfinyl and sulfonyl, provided that atleast one R is not hydrogen.

The term “amino” refers to the group —NH₂. The term “substituted amino”refers to the group —NHR or —NRR where each R is independently selectedfrom the group: optionally substituted acyl, optionally substitutedalkyl, optionally substituted alkoxy, optionally substituted amino,optionally substituted aryl, optionally substituted heteroaryl,optionally substituted heterocyclyl, sulfinyl and sulfonyl, e.g.,methylamino, dimethylamino, diethylamino, methylsulfonylamino,furanyl-oxy-sulfonamino, guanidino.

“Aryl” and “heteroaryl” mean a 5- or 6-membered aromatic ring orheteroaromatic ring containing 1-4 heteroatoms selected from O, N, or S;a bicyclic 9- or 10-membered aromatic ring system or heteroaromatic ringsystem containing 1-4 (or more) heteroatoms selected from O, N, or S; ora tricyclic 13- or 14-membered aromatic ring system or heteroaromaticring system containing 1-4 (or more) heteroatoms selected from O, N, orS. The aromatic 6- to 14-membered carbocyclic rings include, e.g.,benzene, naphthalene, indane, tetralin, and fluorene and the 5- to10-membered aromatic heterocyclic rings include, e.g., imidazole,pyridine, indole, thiophene, benzopyranone, thiazole, furan,benzimidazole, quinoline, isoquinoline, quinoxaline, pyrimidine,pyrazine, tetrazole and pyrazole; particularly imidazole andimidazoline.

“Aralkyl” refers to a residue in which an aryl moiety is attached to theparent structure via an alkyl residue. Examples include benzyl,phenethyl, phenylvinyl, phenylallyl and the like. “Heteroaralkyl” refersto a residue in which a heteroaryl moiety is attached to the parentstructure via an alkyl residue. Examples include furanylmethyl,pyridinylmethyl, pyrimidinylethyl and the like.

The term “aryloxy” refers to the group —O-aryl. Similarly; “aralkoxy”and “heteroaralkoxy” refer, respectively, to an aryl or heteroarylmoiety attached to the parent structure via an alkoxy residue.

“Halogen” or “halo” refers to fluorine, chlorine, bromine or iodine(particularly fluorine, chlorine and bromine). Dihaloaryl, dihaloalkyl,trihaloaryl etc. refer to aryl and alkyl substituted with a plurality ofhalogens, but not necessarily a plurality of the same halogen; thus4-chloro-3-fluorophenyl is within the scope of dihaloaryl.

“Heterocycle” or “heterocyclyl” means a cycloalkyl or aryl residue inwhich one to four of the carbons is replaced by a heteroatom such asoxygen, nitrogen or sulfur (i.e., encompassing heterocydoalkyl andheteroaryl). Examples of heterocyclyl residues that fall within thescope of the invention include imidazolyl, imidazolinyl, pyrrolidinyl,pyrazolyl, pyrrolyl, indolyl, quinolinyl, isoquinolinyl,tetrahydroisoquinolinyl, benzofuranyl, benzodioxanyl, benzodioxolyl(commonly referred to as methylenedioxyphenyl, when occurring as asubstituent), tetrazolyl, morpholinyl, thiazolyl, pyridinyl,pyridazinyl, pyrimidinyl, thiophenyl, furanyl, oxazolyl, oxazolinyl,isoxazolyl, dioxanyl, tetrahydrofuranyl and the like. “N-heterocyclyl”refers to a nitrogen-containing heterocycle as a substituent residue.Examples of N-heterocyclyl residues include 4-morpholinyl,4-thiomorpholinyl, 1-piperidinyl, 1-pyrrolidinyl, 3-thiazolidinyl,piperazinyl and 4-(3,4-dihydrobenzoxazinyl). Examples of substitutedheterocyclyl include 4-methyl-1-piperazinyl and 4-benzyl-1-piperidinyl.

The terms “heteroaryloxy” and “heterocyclooxy” refer, respectively tothe groups —O-heteroaryl and —O-heterocyclyl.

The term “solvate” refers to a compound (e.g., a compound of Formula Ior II or a pharmaceutically acceptable salt thereof) in physicalassociation with one or more molecules of a pharmaceutically acceptablesolvent. It will be understood that phrases such as “a compound ofFormula I or II or a pharmaceutically acceptable salt or solvatethereof” are intended to encompass the compound of Formula I or II, apharmaceutically acceptable salt of the compound, a solvate of thecompound, and a solvate of a pharmaceutically acceptable salt of thecompound.

The term “substituted” as used with regard to alkyl, aryl, aralkyl,heteroaryl and heterocyclyl refers to an alkyl, aryl, aralkyl,heteroaryl or heterocyclyl moiety wherein one or more (up to about 5,particularly up to about 3) hydrogen atoms are replaced by a substituentindependently selected from the group: optionally substituted acyl(e.g., aminocarbonyl and alkoxycarbonyl or “esters”), optionallysubstituted acyloxy (e.g., acid esters, carbamic acid esters, carbonicacid esters, and thiocarbonic acid esters), optionally substituted alkyl(e.g., fluoroalkyl), optionally substituted alkoxy (e.g., methoxy andmethoxymethoxy), alkylenedioxy (e.g. methylenedioxy), optionallysubstituted amino (e.g., alkylamino, dialkylamino, carbonylamino,benzyloxycarbonylamino or “CBZ-amino”, and carboxamido), optionallysubstituted amidino, optionally substituted aryl (e.g., phenyl and4-methyl-phenyl or “tolyl”), optionally substituted aralkyl (e.g.,benzyl), optionally substituted aryloxy (e.g., phenoxy), optionallysubstituted aralkoxy (e.g., benzyloxy), optionally substitutedheteroaryl, optionally substituted heteroaralkyl, optionally substitutedheteroaryloxy, optionally substituted heteroaralkoxy, carboxy (—COOH),cyano, halogen, hydroxy, nitro, sulfanyl, sulfinyl, sulfonyl and thio.In the compounds of Formula II where T is substituted alkylene, the term“substituted” also refers to alkylene groups where one or more (up toabout 3, particularly 1) carbon atoms are replaced by a heteroatomindependently selected from O, N or S, such as —CH₂—S—CH₂—.

The term “sulfanyl” refers to the groups: —S-(optionally substitutedalkyl), —S-(optionally substituted aryl), —S-(optionally substitutedheteroaryl), and —S-(optionally substituted heterocyclyl).

The term “sulfinyl” refers to the groups: —S(O)—H, —S(O)-(optionallysubstituted alkyl), —S(O)-(optionally substituted amino),—S(O)-(optionally substituted aryl), —S(O)-(optionally substitutedheteroaryl), and —S(O)-(optionally substituted heterocyclyl).

The term “sulfonyl” refers to the groups: —S(O₂)—H, —S(O₂)-(optionallysubstituted alkyl), —S(O₂)-(optionally substituted amino),—S(O₂)-(optionally substituted aryl), —S(O₂)-(optionally substitutedheteroaryl), —S(O₂)(optionally substituted heterocyclyl),—S(O₂)-(optionally substituted alkoxy), —S(O₂)-optionally substitutedaryloxy), —S(O₂)-(optionally substituted heteroaryloxy), and—S(O₂)-(optionally substituted heterocyclyloxy).

“Isomers” are different compounds that have the same molecular formula.“Stereoisomers” are isomers that differ only in the way the atoms arearranged in space. “Enantiomers” are a pair of stereoisomers that arenon-superimposable mirror images of each other. A 1:1 mixture of a pairof enantiomers is a “racemic” mixture. The term “(.±.)” is used todesignate a racemic mixture where appropriate. “Diastereoisomers” arestereoisomers that have at least two asymmetric atoms, but which are notmirror-images of each other. The absolute stereochemistry is specifiedaccording to the Cahn-Ingold-Prelog R—S system. When a compound is apure enantiomer the stereochemistry at each chiral carbon may bespecified by either R or S. Resolved compounds whose absoluteconfiguration is unknown are designated (+) or (−) depending on thedirection (dextro- or levorotatory) which they rotate plane polarizedlight at the wavelength of the sodium D line. The term “substantiallypure” means having at least about 95% chemical purity with no singleimpurity greater than about 1%. The term “substantially optically pure”or “substantially enantiomerically pure” means having at least about 95%enantiomeric excess. The invention contemplates the use of pureenantiomers and mixtures of enantiomers, including racemic mixtures,although the use of a substantially optically pure enantiomer willgenerally be most suitable.

“Mitotic spindle formation” refers to the organization of microtubulesinto bipolar structures by mitotic kinesins. “Mitotic spindledysfunction” refers to mitotic arrest, monopolar spindle formation ormitotic spindle malformation, in which context “malformation”encompasses the splaying of mitotic spindle poles, or otherwise causingmorphological perturbation of the mitotic spindle. The term “inhibit” asused with reference to mitotic spindle formation, means altering mitoticspindle formation, including decreasing spindle formation, andincreasing or decreasing spindle pole separation. “Anti-mitotic” meansinhibiting or having the potential to inhibit mitosis, for example, asdescribed above.

The term “pharmaceutically acceptable salts” is meant to include bothacid and base addition salts. A “pharmaceutically acceptable acidaddition salt” refers to those salts that retain the biologicaleffectiveness of the free bases and that are not biologically orotherwise undesirable, formed with inorganic acids such as hydrochloricacid, hydrobromic acid, sulfuric acid, nitric acid, phosphoric acid andthe like, or organic acids such as acetic acid, propionic acid, glycolicacid, pyruvic acid, oxalic acid, maleic acid, malonic acid, succinicacid, fumaric acid, tartaric acid, citric acid, benzoic acid, cinnamicacid, mandelic acid, methanesulfonic acid, ethanesulfonic acid,p-toluenesulfonic acid, salicylic acid and the like. “Pharmaceuticallyacceptable base addition salts” include those derived from inorganicbases such as sodium, potassium, lithium, ammonium, calcium, magnesium,iron, zinc, copper, manganese, aluminum salts and the like. Particularlysuitable are the ammonium, potassium, sodium, calcium, and magnesiumsalts. Salts derived from pharmaceutically acceptable organic non-toxicbases include salts of primary, secondary, and tertiary amines,substituted amines including naturally occurring substituted amines,cyclic amines and basic ion exchange resins, such as isopropylamine,trimethylamine, diethylamine, triethylamine, tripropylamine, andethanolamine.

The term “therapeutically effective amount” or “effective amount” refersto that amount of a compound of Formula I or II that is sufficient toeffect treatment, as defined below, when administered to a patient inneed of such treatment. The effective amount will vary depending uponthe patient and disease condition being treated, the weight and age ofthe patient, the severity of the disease condition, the particularcompound, pharmaceutically acceptable salt or solvate of Formula I or IIchosen, the dosing regimen to be followed, timing of administration, themanner of administration and the like, all of which can readily bedetermined by one of ordinary skill in the art. In a particular aspectof the invention, the effective amount will be an amount sufficient toinhibit KSP kinesin activity in cells involved with the disease beingtreated.

The term “treatment” or “treating” means any treatment of a disease in apatient, including:

-   -   a) preventing the disease, that is, causing the clinical        symptoms of the disease not to develop;    -   b) inhibiting the disease, that is, slowing or arresting the        development of clinical symptoms; and/or    -   c) relieving the disease, that is, causing the regression of        clinical symptoms.

A “patient” for the purposes of the present invention includes humansand other animals, particularly mammals, and other organisms. Thus themethods are applicable to both human therapy and veterinaryapplications. In a particular embodiment the patient is a mammal, mostparticularly the patient is human.

COMPOUNDS OF THE PRESENT INVENTION

The present invention provides certain quinazolinone derivatives. Thecompounds are inhibitors of one or more mitotic kinesins. The presentinvention capitalizes on the finding that perturbation of mitotickinesin function causes malformation or dysfunction of mitotic spindles,frequently resulting in cell cycle arrest and cell death.

Accordingly, the present invention relates to one or more compoundsselected from the group represented by Formula I:

where:

-   -   U—V is chosen from —N(R⁶)—CR^(e)R^(f)—, —CR^(e)R^(f)—N(R⁶)—,        N(R⁶)—CR^(e)R^(f)—CR⁹R^(h)—, —CR^(e)R^(f)—N(R⁶)—CR⁹R^(h)—, and        —CR^(e)R^(f)—CR⁹R^(h)—N(R⁶)—;    -   R^(a), R^(b), R^(c), R^(d), R^(e), R^(f), R^(g) and R^(h) are        independently chosen from hydrogen, alkyl, aryl, aralkyl,        heteroaryl, substituted alkyl, substituted aryl, substituted        aralkyl and substituted heteroaryl;    -   R¹, R², R³, and R⁴ are independently chosen from hydrogen,        alkyl, alkoxy, halogen, cyano and substituted alkyl;    -   R⁵ is chosen from alkyl, aryl, aralkyl, heteroaryl,        heteroaralkyl, substituted alkyl, substituted aryl, substituted        aralkyl, substituted heteroaryl and substituted heteroaralkyl;        and    -   R⁶ is chosen from hydrogen, acyl, alkyl, aryl, aralkyl,        heteroaryl, substituted acyl, substituted alkyl, substituted        aryl, substituted aralkyl and substituted heteroaryl;        induding single stereoisomers, mixtures of stereoisomers, and        pharmaceutically acceptable salts, solvates, and solvates of        pharmaceutically acceptable salts thereof.

In another aspect, the invention relates to one or more compoundsselected from the group represented by Formula II:

where:

-   -   T is a covalent bond or optionally substituted lower alkylene;    -   W, X and Y are independently chosen from —N═, N, —C═, CH,        CR^(i), O and S;    -   Z is chosen from —N═, N, —C═, CH, CR^(i) or is absent, provided        that:        -   no more than two of W, X, Y and Z are —N═, and        -   W, X or Y can be O or S only when Z is absent;    -   R^(i) is chosen from alkyl, alkoxy, halogen, cyano and        substituted alkyl; and    -   R¹ to R⁶, U and V are as defined with regard to Formula I,        provided that R¹, R², R³ or R⁴ is absent where W, X, Y or Z,        respectively, is —N═, O, S or absent;        including single stereoisomers, mixtures of stereoisomers, and        the pharmaceutically acceptable salts, solvates, and solvates of        pharmaceutically acceptable salts thereof. The compounds        encompassed by Formula II will be seen to include those of        Formula I; they are likewise useful as active agents in the        practice of the methods of treatment and in manufacture of        compositions including the pharmaceutical formulations of the        invention, and may also be useful as intermediates in the        synthesis of such active agents. The dashed lines in Formula II        indicate that the corresponding bond may be a single bond (e.g.,        where X is CH) or a double bond (e.g., where X is C═). For the        sake of simplicity in the following description and claims,        substituents T, U, W, X, Y and Z will not be discussed in        connection with certain compounds falling within the scope of        Formula I.

Many of the compounds described herein contain one or more asymmetriccenters and may thus give rise to enantiomers, diastereomers, and otherstereoisomeric forms that may be defined, in terms of absolutestereochemistry, as (R)— or (S)—. When the compounds described hereincontain olefinic double bonds or other centers of geometric asymmetry,and unless specified otherwise, it is intended that such compoundsinclude both E and Z geometric isomers. All tautomeric forms are alsointended to be included. The present invention is meant to include allsuch possible isomers, including racemic mixtures, intermediatemixtures, optically pure forms, substantially optically pure forms,enantiomerically pure forms, and substantially enantiomerically pureforms.

Nomenclature

The compounds of Formula I and II can be named and numbered (e.g., usingAutoNom version 2.1 in ISIS-DRAW or ChemDraw) as described below.

For example, the compound of Formula IA:

i.e., the compound according to Formula I where U—V is—N(R⁶)—CR^(e)R^(f)—; R¹, R², R⁴, and R^(a) to R^(f) are H; R³ is chloro;R⁵ is benzyl; and R⁶ is p-methyl-benzoyl, can be named3-benzyl-7-chloro-2-[1-(4-methyl-benzoyl)-pyrrolidin-2-yl]-3H-quinazolin-4-one.

The compound of Formula IB:

i.e., the compound according to Formula I where U—V is—N(R⁶)—CR^(e)R^(f)—CR^(g)R^(h)—; R¹, R², R⁴ and R^(a) to R^(h) are H; R³is chloro; R⁵ is benzyl; and R⁶ is p-methyl-benzoyl, can be named3-benzyl-7-chloro-2-[1-(4-methyl-benzoyl)-piperidin-2-yl]-3H-quinazolin-4-one.

The compound of Formula IC:

i.e., the compound according to Formula I where U—V is—CR^(e)R^(f)—N(R⁶)—CR^(g)R^(h)—; R¹, R⁴, and R^(a) to R^(h) are H; R²and R³ are methoxy; R⁵ is benzyl; and R⁶ is p-methyl-benzyl, can benamed3-benzyl-6,7-dimethoxy-2-[1-(4-methyl-benzyl)-piperidin-3-yl]-3H-quinazolin-4-one.

The compound of Formula ID:

i.e., the compound according to Formula I where U—V is—CR^(e)R^(f)—CR^(g)R^(h)—N(R⁶)—; R¹, R², R⁴, R^(b) and R^(d) to R^(h)are H; R³ is chloro; R⁵ is methyl; R^(a) is phenethyl; R^(c) isdiethylamino-ethyl; and R⁶ is p-methyl-benzoyl, can be named7-chloro-2-[2-(2-diethylamino-ethyl)-1-(4-methyl-benzoyl)-3-phenethyl-piperidin-4-yl]-3-methyl-3H-quinazolin-4-one.

The compound of Formula IIA:

i.e., the compound according to Formula II where T is methylene; U—V is—N(R⁶)—CR^(e)R^(f)—; W, Y and Z are —C═; X is —N═; R¹, R⁴ and R^(a) toR^(f) are H; R² is absent; R³ is chloro; R⁵ is benzyl; and R⁶ isp-methyl-benzoyl, can be named3-benzyl-7-chloro-2-[1-(4-methyl-benzoyl)-pyrrolidin-2-ylmethyl]-3H-pyrido[4,3-d]pyrimidin-4-one.

The compound of Formula IIB:

i.e., the compound according to Formula I where the dashed line betweenW and X is a single bond, T is 3-propionyl; U—V is—N(R⁶)—CR^(e)R—CR^(g)R^(h)—; W is N; X is CR^(i) where R^(i) is methyl;Y is CH; Z is ═N—; R¹, R⁴ and R^(a) to R^(h) are H; R² is methyl; R³ isabsent; R⁵ is benzyl; and R⁶ is p-methyl-benzoyl, can be named3-benzyl-6,6-dimethyl-2-3-[1-(4-methyl-benzoyl)-piperidin-2-yl]-propionyl}-5,6-dihydro-3H-pteridin-4-one.

The compound of Formula IIC:

i.e., the compound according to Formula II where the dashed lines aresingle bonds, T is absent; U—V is —CR^(e)R^(f)—N(R⁶)—CR^(g)R^(h); Z, R²and R⁴ are absent; W and Y are CH; X is S; R¹, R³, and R^(a), R^(b), andR^(d) to R^(h) are H; R^(c) is isopropyl; R⁵ is benzyl; and R⁶ isp-methyl-benzyl, can be named3-benzyl-2-[5-isopropyl-1-(4-methyl-benzyl)-piperidin-3-yl]-5,7-dihydro-3H-thieno[3,4-d]pyrimidin-4-one.

The compound of Formula IID:

i.e., the compound according to Formula II where T is aminomethylene;U—V is —CR^(e)R^(f)—CR^(g)R^(h)—N(R⁶)—; R¹, R², R⁴, R^(b) and R^(d) toR^(h) are H; R³ is chloro; R⁵ is methyl; R^(a) is benzyl; R^(c) isdiethylamino-ethyl; and R⁶ is p-carbonyl-benzamide, can be named4-[3-benzyl-4-[(7-chloro-3-methyl-4-oxo-3,4-dihydro-quinazolin-2-ylmethyl)-amino]-2-(2-diethylamino-ethyl)-piperidine-1-carbonyl-benzamide.Synthetic Reaction Parameters

The terms “solvent”, “inert organic solvent” or “inert solvent” mean asolvent inert under the conditions of the reaction being described inconjunction therewith [including, for example, benzene, toluene,acetonitrile, tetrahydrofuran (“THF”), dimethylformamide (“DMF”),chloroform, methylene chloride (or dichloromethane), diethyl ether,methanol, pyridine and the like]. Unless specified to the contrary, thesolvents used in the reactions of the present invention are inertorganic solvents.

The term “q.s.” means adding a quantity sufficient to achieve a statedfunction, e.g., to bring a solution to the desired volume (i.e., 100%).

Isolation and purification of the compounds and intermediates describedherein can be effected, if desired, by any suitable separation orpurification procedure such as, for example, filtration, extraction,crystallization, column chromatography, thin-layer chromatography orthick-layer chromatography, or a combination of these procedures.Specific illustrations of suitable separation and isolation procedurescan be had by reference to the examples hereinbelow. However, otherequivalent separation or isolation procedures can, of course, also beused.

When desired, the (R)— and (S)-isomers may be resolved by methods knownto those skilled in the art, for example by formation ofdiastereoisomeric salts or complexes which may be separated, forexample, by crystallisation; via formation of diastereoisomericderivatives which may be separated, for example, by crystallisation,gas-liquid or liquid chromatography; selective reaction of oneenantiomer with an enantiomer-specific reagent, for example enzymaticoxidation or reduction, followed by separation of the modified andunmodified enantiomers; or gas-liquid or liquid chromatography in achiral environment, for example on a chiral support, such as silica witha bound chiral ligand or in the presence of a chiral solvent. Forexample, a compound of Formula I or II can be dissolved in a loweralkanol and placed on a Chiralpak AD (205×20 mm) column (ChiralTechnologies, Inc.) conditioned for 60 min at 70% EtOAc in Hexane. Itwill be appreciated that where the desired enantiomer is converted intoanother chemical entity by one of the separation procedures describedabove, a further step may be required to liberate the desiredenantiomeric form. Alternatively, specific enantiomer may be synthesizedby asymmetric synthesis using optically active reagents, substrates,catalysts or solvents, or by converting one enantiomer to the other byasymmetric transformation.

Synthesis of the Compounds of Formula I and II

Brief Description of Reaction Schemes

Syntheses of the compounds of Formula I and II are described below withreference to Reaction Schemes 1 and 2.

Reaction Scheme 1 illustrates a synthesis of the compounds of Formulae Iand II [excluding the 2-(piperidin-2-yl)-3-quinazolin-4-ones]. Thenitrogen atom of U—V is protected until cyclization, followed byde-protection and derivatization to provide R⁶ substituents other thanhydrogen.

Reaction Scheme 2 depicts a synthesis for the compounds of Formulae Iand II [including 2-(piperidin-2-yl)-3H-quinazolin-4-ones], illustratingthe synthesis of compounds where R⁶ is acyl.

It will be appreciated by those skilled in the art that one or more ofthe reactants, steps and/or conditions described with reference toReaction Schemes 1 and 2 may require adjustment to accommodatenon-hydrogen substituents, e.g., at R¹ to R⁶ and R^(a) to R^(h).

Starting Materials

The N-protected pyrrolidine and piperidine dicarboxylic acids of Formula101 (e.g., pyrrolidine-1,2-dicarboxylic acid 1-tert-butyl ester), theanthranilic acids of Formula 102 (e.g., 4-chloro-anthranilic acid) andother reactants are commercially available, e.g., from Aldrich ChemicalCompany, Milwaukee, Wisc. or may be readily prepared by those skilled inthe art using commonly employed synthetic methodology.

Referring to Reaction Scheme 1, the R⁶ component of “U—V” is notspecifically shown. In Formulae 102 to 106 the R⁶ moiety is a protectinggroup. In the end products of Step 5 (Formula I or II) R⁶ is as defined,initially being hydrogen upon de-protection of U—V and optionally beingfurther derivatized. Products where R⁶ is aryl or heteroaryl can beobtained by starting with the corresponding arylated compound of Formula101 or by a palladium catalyzed arylation (e.g., as described by Wolfe,J. P.; Tomori, H; Sadighi, J. P.; Yin, J.; and Buchwald, S. L., J. Org.Chem., 2000, 65, 1158-1174) of a de-protected product of Step 5 where R⁶is hydrogen.

Preparation of Formula 103 Referring to Reaction Scheme 1, Step 1, asolution is made of an optionally substituted o-amino alicyclic,heterocyclic or (hetero)aryl acid of Formula 102 (such as anthranilicacid), a slight molar excess of an N-protected pyrrolidine- orpiperidine-dicarboxylic acid of Formula 101 [where U′—V′ is—N(R^(6′))—CR^(e)R^(f)—, —CR^(e)R^(f)—N(R^(6′))—,—CR^(e)R^(f)—N(R^(6′))—CR^(g)R^(h)— or —CR^(e)R^(f)—CR^(g)R^(h)—N(R⁶)—,and R^(6′) is an amino-protecting group, such as Fmoc and Boc] and aslight molar excess of PyBroP dissolved in an organic solvent (e.g.,diisopropylethylamine in pyridine). The solution is stirred for 12 to 20hours at room temperature to afford the corresponding compound ofFormula 103, which is conventionally isolated and purified.

Preparation of Formula 104 Referring to Reaction Scheme 1, Step 2, acompound of Formula 103 is dissolved in an organic solvent (e.g., alower alkanol such as ethanol) and treated with an aqueous hydroxide(e.g., NaOH aq. solution). The mixture is stirred at room temperaturefor 12 to 20 hours and then concentrated under vacuum. The residue iswashed (e.g., with saturated NaCl and concentrated phosphoric acid) andthen extracted (e.g., with dichloromethane). Conventional isolation andpurification affords the corresponding acid of Formula 104.

Preparation of Formula 105 Referring to Reaction Scheme 1, Step 3, to asolution of Formula 104 in an organic solvent (e.g., dichloromethane) isadded large molar excesses of EDC and diisopropylethylamine. Afterstirring at room temperature for 1 to 2 hours, a large molar excess ofan amine of the formula H₂N—R⁵ (such as benzylamine) is added and thereaction is stirred for an additional 24 to 60 hours. The mixture iswashed, dried and isolated to afford the corresponding di-carbamoylcompound of Formula 105, and may include a small amount of thecorresponding cyclized compound of Formula 106, which is taken forwardwithout further purification.

Preparation of Formula 106 Referring to Reaction Scheme 1, Step 4, acompound of Formula 105 (optionally in the presence of a compound ofFormula 106) is stirred in ethylene glycol with K₂CO₃ at 120° C. for 12to 20 hours, allowed to cool to room temperature, and is extracted(e.g., with dichloromethane). The organic fractions are conventionallyisolated and purified to afford the pure cyclized compound of Formula106.

Preparation of Formula I or II where R⁶ is Hydrogen Referring toReaction Scheme 1, Step 5, a solution of Formula 106 where R^(6′) ofU′—V′ is a Boc group, is treated with a 1:1 mixture ofTFA/dichloromethane for 30 minutes to 2 hours at room temperature. Thesolution is concentrated under vacuum and partitioned (e.g., betweendichloromethane and saturated NaHCO₃). The aqueous layer is extracted(e.g., with dichloromethane) and the combined organic layers areconventionally isolated to afford the corresponding de-protectedpyrrolidinyl- or piperidinyl product of Formula I or II, which can bepurified or taken forward without further purification.

Preparation of Formula I or II where R⁶ is Acyl To a solution of FormulaI or II where R⁶ is hydrogen in an organic solvent (e.g,.dichloromethane) is added large molar excesses of an acyl halide (e.g.,toluoyl chloride) and diisopropylethylamine. The mixture is stirred for30 minutes to 2 hours and then partitioned (e.g., between saturatedNaHCO₃ and ethyl acetate). Conventional isolation and purification ofthe organic phase affords the corresponding compound of Formula I or IIwhere R⁶ is acyl.

Preparation of Formula I where R⁶ is Optionally Substituted Alkyl,Optionally Substituted Aralkyl or Optionally Sutstituted HeteroaralkylTo a solution of Formula I or II where R⁶ is hydrogen in an organicsolvent (e.g,. dichloromethane) is added a slight molar excess of analdehyde comprising R⁶⁻ (i.e., a compound having formula R⁶⁻—CHO whereR⁶⁻—CH₂— is equivalent to R⁶ and R⁶ is as described above; e.g.,fptolualdehyde) and a large molar excess of NaHB(OAc)₃. The mixture isstirred for 30 minutes to 1 hour at room temperature. The reaction isquenched with saturated NaHCO₃ and the aqueous phase extracted (e.g.,with dichloromethane). Conventional isolation and purification of theorganic phase affords the corresponding compound of Formula I or IIwhere R⁶ is optionally substituted-alkyl, -aralkyl or -heteroaralkyl.

Referring to Reaction Scheme 2, in Formula 201 the R⁶ moiety of “U—V” ishydrogen. In Formulae 202 through 205 and in Formulae I and II, the“N-Acyl” moiety represents the —N(R⁶)— portion of “U—V” where R⁶ isAcyl.

Preparation of Formula 202 Referring to Reaction Scheme 2, Step 1, aslight molar excess of an acyl halide (e.g., p-toluoyl chloride) isslowly added, portionwise, to a 0° C. solution of a pyrollidine- orpiperidine-mrboxylic acid of Formula 201 in 2M NaOH. The mixture isstirred for an additional 30 minutes to 2 hours at room temperature,cooled to 0° C., and partitioned (e.g., between 1N HCl anddichloromethane). The organic layer is isolated and purified to affordthe corresponding N-acyl-pyrollidine- or -piperidine-carboxylic acid ofFormula 202.

Preparation of Formula 203 Referring to Reaction Scheme 2, Step 2, acompound of Formula 102 is combined with a slight molar excess of acompound of Formula 202, and one-fifth molar equivalents each of PyBroPand diisopropylethylamine in DMF. The reaction takes place at −15° C.with stirring for 12 to 20 hours, allowing the temperature to rise toroom temperature. The product is isolated and purified to afford thecorresponding compound of Formula 203.

Preparation of Formula 205 Referring to Reaction Scheme 2, Steps 3 and4, a mixture of a compound of Formula 203 and 1 M NaOH in a mixedsolvent (e.g., methanol and dioxane) is stirred at room temperature for5 to 10 hours. The solvents are evaporated and the residue ispartitioned (e.g., between dichloromethane, saturated NaCl, andconcentrated HCl). The layers are separated and the organic layer driedand concentrated under vacuum to afford the corresponding compound ofFormula 204, which crude product is carried forward without furtherpurification. The crude product is redissolved (e.g., indichloromethane) and treated with a large molar excess of both EDC anddiisopropylethylamine. After stirring for 10 to 30 minutes, a largemolar excess of an amine of the formula H₂N—R⁵ (such as benzylamine) isadded and the mixture stirred for 12 to 20 hours at room temperature.The mixture is washed, dried and isolated to afford the correspondingcrude di-carbamoyl compound of Formula 205, which can be taken forwardwithout further purification.

Prerparation of Formula I or II Referring to Reaction Scheme 2, Step 5,a mixture of a compound of Formula 205 and a large molar excess of K₂CO₃in a suitable solvent (e.g., ethylene glycol) is stirred at 120° C. for12 to 20 hours. The solution is cooled to room temperature, diluted withwater, and extracted (e.g., with dichloromethane and ethyl acetate). Thecombined organic layers are washed, isolated and purified to afford thecorresponding 1-(acyl)-pyrrolidinyl- or 1-(acyl)-piperidinyl- product ofFormula I or II.

Compounds prepared by the above-described process of the invention maybe identified by the presence of a detectable amount of Formulae 105 or205, or a protected precursor of Formula 106. While it is well knownthat pharmaceuticals must meet pharmacopoeia standards before approvaland/or marketing, and that synthetic reagents (such as benzylamine,ethylene glycol or NaOH) and precursors (such as Formulae 105, 106 and205) should not exceed the limits prescribed by pharmacopoeia standards,final compounds prepared by a process of the present invention may haveminor, but detectable, amounts of such materials present, for example atlevels in the range of 95% purity with no single impurity greater than1%. These levels can be detected, e.g., by emission spectroscopy. It isimportant to monitor the purity of pharmaceutical compounds for thepresence of such materials, which presence is additionally disclosed asa method of detecting use of a process of the invention.

Particular Optional Processes and Last Steps

A compound of Formula 205 is cyclized under basic conditions to afford acorresponding, optionally protected2-[1-(acyl)-pyrrolidinyl]-3H-quinazolin-4-one or2-[1-(acyl)-piperidinyl]-3-quinazolin-4-one of Formula I.

A protected precursor to Formula I or II (e.g., Formula 106 where R⁶ isNHBoc) is de-protected with in TFA in a suitable solvent to afford thecorresponding de-protected compound of Formula I or II.

A compound of Formula I or II where R⁶ is hydrogen is contacted with analkyl or aryl halide, aldehyde or acid chloride in a suitable solvent toafford the corresponding compound of Formula I or II where R⁶ is otherthan hydrogen.

A racemic mixture of isomers of a compound of Formula I or II is placedon a chromatography column and separated into (R)— and (S)-enantiomers.

A compound of Formula I or II is contacted with a pharmaceuticallyacceptable acid to form the corresponding acid addition salt.

A pharmaceutically acceptable acid addition salt of Formula I or II iscontacted with a base to form the corresponding free base of Formula Ior II.

Particular Compounds

Particular embodiments of the invention include or employ the compoundsof Formulae I and II having the following combinations and permutationsof substituent groups (indented/sub-grouped, respectively, in increasingorder of particularity). These are presented in support of the appendedclaims as well as combinations and permutations of substituent groupsthat may, for the sake of brevity, not be specifically claimed butshould be appreciated as encompassed by the teachings of the presentdisclosure. In that regard, the below-described subsets for eachsubstituent (sometimes referenced by paragraph number) are intended toapply to that substituent alone or in combination with one, several, orall of the described subsets for the other substituents.

-   W, X, Y and Z are independently chosen from —C═ and —N═.    -   W, X, Y and Z are —C═.-   R¹, R², R³ and R⁴ are independently chosen from hydrogen, halo,    lower alkyl, substituted lower alkyl, lower alkoxy, and cyano.    -   R¹, R², R³ and R⁴ are independently hydrogen, chloro, fluoro,        methyl, methoxy, cyano or substituted lower alkyl.        -   R¹, R², R³ and R⁴ are independently hydrogen, chloro,            fluoro, methyl, methoxy or cyano.    -   Where three or four of R¹, R², R³ and R⁴ are hydrogen.        -   R¹, R², R³ and R⁴ are hydrogen or three of R¹, R², R³ and R⁴            are hydrogen and the fourth is halo, methoxy, methyl, or            cyano.            -   Where halo is chloro.                -   Where R³ is hydrogen or chloro.                -    Where R³ is chloro.            -   Where R¹, R², R³′ and R⁴ are hydrogen.

R⁵ is optionally substituted aralkyl.

-   -   R⁵ is benzyl or substituted benzyl.        -   R⁵ is benzyl.

T is optionally substituted lower alkylene or is a covalent bond (i.e.,absent).

-   -   T is a covalent bond, C₁ to C₄ alkylene or C₁ to C₄ alkylene        substituted with halo or oxo.        -   T is a covalent bond or C₁ to C₄ alkylene.            -   T is a covalent bond.    -   Where T is alkylene having a carbon substituted by a heteroatom,        the heteroatom is not bound directly to the bicyclic structure.        -   T is aminoalkylene or amidoalkylene.    -   T is alkylene or alkylene substituted with halo or oxo.

R^(a), R^(b), R^(c), R^(d), R^(e), R^(f), R^(g) and R^(h) areindependently chosen from hydrogen, lower alkyl (especially methyl), andsubstituted lower alkyl.

-   -   Where no more than one of R^(a) to R^(h) is other than hydrogen.        -   Where all of R^(a) to R^(h) are hydrogen.

-   U—V is —N(R⁶)—CR^(e)R^(f)—CR^(g)R^(h)—,    CR^(e)R^(f)—N(R⁶)—CR^(g)R^(h)—, or —CR^(e)R^(f)—CR^(g)R^(h)—N(R⁶)—.    -   U—V is —N(R⁶)CR^(e)R^(f)—CR^(g)R^(h) or        —CR^(e)R^(f)—N(R⁶)—CR^(g)R^(h)—.        -   Where R⁶ is optionally substituted aralkyl or optionally            substituted acyl.            -   Where R⁶ is optionally substituted acyl.                -   Where R⁶ is p-methyl-benzoyl.        -   U—V is —N(R⁶)—CR^(e)R—CR^(g)R^(h).            -   Where R⁶ is optionally substituted aralkyl or optionally                substituted acyl.                -   Where R⁶ is optionally substituted acyl.                -    Where R⁶ is p-methyl-benzoyl.

Illustrative of the suitable combinations and permutations of particularsubstituents are the compounds, pharmaceutically acceptable salts andsolvates where T is a covalent bond, U—V is—N(R⁶)—CR^(e)R^(f)—CR^(g)R^(h), and one or more of W, X, Y and Z, R¹ toR⁶, and R^(a) to R^(h) is/are as described in paragraphs 089 to 094above, such as:

-   Where R¹, R², R³ and R⁴ are hydrogen or three of R¹, R², R³ and R⁴    are hydrogen and the fourth is halo, methoxy, methyl, or cyano.    -   Where R³ is chloro.    -   Where R¹, R², R³ and R⁴ are hydrogen.-   Where R⁵ is optionally substituted benzyl.    -   R⁵ is benzyl.-   Where all but one of R^(a), R^(b), R^(c), R^(d), R^(e), R^(f), R^(g)    and R^(h) are hydrogen, and the remaining member is chosen from    hydrogen, lower alkyl (especially methyl) and substituted lower    alkyl.    -   All of R^(a) to R^(h) are hydrogen.-   Where R⁶ is optionally substituted acyl.    -   R⁶ is p-methyl-benzoyl.        -   Where all but one of R^(a), R^(b), R^(c), R^(d), R^(e),            R^(f), R^(g) and R^(h) are hydrogen, and the remaining            member is chosen from hydrogen, lower alkyl (especially            methyl) and substituted lower alkyl.            -   All of R^(a) to R^(h) are hydrogen.                -   Where R⁵ is optionally substituted benzyl.                -    R⁵ is benzyl.                -    Where R¹, R², R³ and R⁴ are hydrogen or three of                    R¹, R², R³ and R⁴ are hydrogen and the fourth is                    halo, methoxy, methyl, or cyano.                -    Where R³is chloro.                -    Where R¹, R², R³ and R⁴ are hydrogen.                -    Where R¹, R², R³ and R⁴ are hydrogen or three of                    R¹, R², R³ and R⁴ are hydrogen and the fourth is                    halo, methoxy, methyl, or cyano.                -    Where R³is chloro.                -    Where R¹, R², R³ and R⁴ are hydrogen.                -   Where R¹, R², R³ and R⁴ are hydrogen or three of R¹,                    R², R³ and R⁴ are hydrogen and the fourth is halo,                    methoxy, methyl, or cyano.                -    Where R³ is chloro.                -    Where R¹, R², R³ and R⁴ are hydrogen.            -   Where R⁵ is optionally substituted benzyl.                -   R⁵ is benzyl.                -    Where R¹, R², R³ and R⁴ are hydrogen or three of                    R¹, R², R³ and R⁴ are hydrogen and the fourth is                    halo, methoxy, methyl, or cyano.                -    Where R³ is chloro.                -    Where R¹, R², R³ and R⁴ are hydrogen.                -   Where R¹, R², R³ and R⁴ are hydrogen or three of R¹,                    R², R³ and R⁴ are hydrogen and the fourth is halo,                    methoxy, methyl, or cyano.                -    Where R³ is chloro.                -    Where R¹, R², R³ and R⁴ are hydrogen.            -   Where R¹, R², R³ and R⁴ are hydrogen or three of R¹, R²,                R³ and R⁴ are hydrogen and the fourth is halo, methoxy,                methyl, or cyano.                -   Where R³is chloro.                -   Where R¹, R², R³ and R⁴ are hydrogen.        -   Where R⁵ is optionally substituted benzyl.            -   R⁵ is benzyl.            -   Where R¹, R², R³ and R⁴ are hydrogen or three of R¹, R²,                R³ and R⁴ are hydrogen and the fourth is halo, methoxy,                methyl, or cyano.                -   Where R³is chloro.                -   Where R¹, R², R³ and R⁴ are hydrogen.        -   Where R¹, R², R³ and R⁴ are hydrogen or three of R¹, R², R³            and R⁴ are hydrogen and the fourth is halo, methoxy, methyl,            or cyano.            -   Where R³is chloro.            -   Where R¹, R², R³ and R⁴ are hydrogen.    -   Where all but one of R^(a), R^(b), R^(c), R^(d), R^(e), R^(f),        R^(g) and R^(h) are hydrogen, and the remaining member is chosen        from hydrogen, lower alkyl (especially methyl) and substituted        lower alkyl.        -   All of R^(a) to R^(h) are hydrogen.            -   Where R⁵is optionally substituted benzyl.                -   R⁵is benzyl.                -    Where R¹, R², R³ and R⁴ are hydrogen or three of                    R¹, R², R³ and R⁴ are hydrogen and the fourth is                    halo, methoxy, methyl, or cyano.                -    Where R³is chloro.                -    Where R¹, R², R³ and R⁴ are hydrogen.                -   Where R¹, R², R³ and R⁴ are hydrogen or three of R¹,                    R², R³ and R⁴ are hydrogen and the fourth is halo,                    methoxy, methyl, or cyano.                -    Where R³is chloro.                -    Where R¹, R², R³ and R⁴ are hydrogen.            -   Where R¹, R², R³ and R⁴ are hydrogen or three of R¹, R²,                R³ and R⁴ are hydrogen and the fourth is halo, methoxy,                methyl, or cyano.                -   Where R³is chloro.                -   Where R¹, R², R³ and R⁴ are hydrogen.        -   Where R⁵ is optionally substituted benzyl.            -   R⁵is benzyl.                -   Where R¹, R², R³ and R⁴ are hydrogen or three of R¹,                    R², R³ and R⁴ are hydrogen and the fourth is halo,                    methoxy, methyl, or cyano.                -    Where R³is chloro.                -    Where R¹, R², R³ and R⁴ are hydrogen.            -   Where R¹, R², R³ and R⁴ are hydrogen or three of R¹, R²,                R³ and R⁴ are hydrogen and the fourth is halo, methoxy,                methyl, or cyano.                -   Where R³ is chloro.                -   Where R¹, R², R³ and R⁴ are hydrogen.        -   Where R¹, R², R³ and R⁴ are hydrogen or three of R¹, R², R³            and R⁴ are hydrogen and the fourth is halo, methoxy, methyl,            or cyano.            -   Where R³is chloro.            -   Where R¹, R², R³ and R⁴ are hydrogen.    -   Where R⁵ is optionally substituted benzyl.        -   R⁵ is benzyl.            -   Where R¹, R², R³ and R⁴ are hydrogen or three of R¹, R²,                R³ and R⁴ are hydrogen and the fourth is halo, methoxy,                methyl, or cyano.                -   Where R³is chloro.                -   Where R¹, R², R³ and R⁴ are hydrogen.                -   Where R¹, R², R³ and R⁴ are hydrogen or three of R¹,                    R², R³ and R⁴ are hydrogen and the fourth is halo,                    methoxy, methyl, or cyano.            -   Where R³is chloro.            -   Where R¹, R², R³ and R⁴ are hydrogen.    -   Where R¹, R², R³ and R⁴ are hydrogen or three of R¹, R², R³ and        R⁴ are hydrogen and the fourth is halo, methoxy, methyl, or        cyano.        -   Where R³ is chloro.        -   Where R¹, R², R³ and R⁴ are hydrogen.

Thus, the compounds where T is a covalent bond and U—V is—N(R⁶)—CR^(e)R^(f)—CR^(g)R^(h), including those where theabove-described groupings and sub-groups of substituents are takenindividually and/or combined together as illustrated with regard tothose compounds where R⁶ is optionally substituted acyl, areparticularly suitable for practice of the present invention.

One group of compounds, pharmaceutically acceptable salts and solvatesthereof, compositions including pharmaceutical formulations, and methodsof manufacture and use of the present invention are those wherein thecompound of Formula I or II is selected from:

-   3-benzyl-7-chloro-2-[1-(4-methyl-benzyl)-pyrrolidin-2-yl]-3H-quinazolin-4-one;-   3-benzyl-7-chloro-2-[1-(4-methyl-benzoyl)-pyrrolidin-2-yl]-3H-quinazolin-4-one;-   3-benzyl-7-chloro-2-[1-(4-methyl-benzoyl)-piperidin-2-yl]-3H-quinazolin-4-one;-   3-benzyl-7-chloro-2-[1-(4-methyl-benzyl)-piperidin-3-yl]-3H-quinazolin-4-one;-   3-benzyl-7-chloro-2-[1-(4-methyl-benzoyl)-piperidin-3-yl]-3H-quinazolin-4-one;-   3-benzyl-7-chloro-2-[1-(4-methyl-benzyl)-piperidin-4-yl]-3H-quinazolin-4-one;    and-   3-benzyl-7-chloro-2-[1-(4-methyl-benzoyl)-piperidin-4-yl]-3H-quinazolin-4-one,    especially the (R)-enantiomers thereof.

A particular group of compounds, pharmaceutically acceptable salts andsolvates thereof, compositions including pharmaceutical formulations,and methods of manufacture and use of the present invention are thosewherein the compound of Formula I or II is selected from:

-   3-benzyl-7-chloro-2-[1-(4-methyl-benzoylypyrrolidin-2-yl]-3H-quinazolin-4-one;-   3-benzyl-7-chloro-2-[1-(4-methyl-benzoylypiperidin-2-yl]-3H-quinazolin-4-one;-   3-benzyl-7-chloro-2-[1-(4-methyl-benzoyl)-piperidin-3-yl]-3H-quinazolin-4-one;    and-   3-benzyl-7-chloro-2-[1-(4methyl-benzoyl)-piperidin-4-yl]-3H-quinazolin-4-one,    especially the (R)-enantiomers thereof.

Another particular group of compounds, pharmaceutically acceptable saltsand solvates thereof, compositions including pharmaceuticalformulations, and methods of manufacture and use of the presentinvention are those wherein the compound of Formula I or 11 is selectedfrom:

-   3-benzyl-7-chloro-2-[1-(4-methyl-benzoyl)-piperidin-2-yl]-3H-quinazolin-4-one;    and-   3-benzyl-7-chloro-2-[1-(4-methyl-benzoylypiperidin-3-yl]-3H-quinazolin-4-one,    especially the (R)-enantiomers thereof.

Utility, Testing and Administration

General Utility

The compounds of the invention find use in a variety of applications,including as therapeutic active agents, in the practice of the methodsof treatment, in compositions, particularly pharmaceutical formulationsand in methods for the manufacture of pharmaceutical formulations, andas intermediates in the synthesis of such therapeutic active agents.

As will be appreciated by those in the art, mitosis can be altered in avariety of ways; that is, one can affect mitosis either by increasing,decreasing or otherwise interfering with the activity of a component inthe mitotic pathway. Stated differently, mitosis can be affected (e.g.,disrupted) by disturbing equilibrium, either by inhibiting or activatingcertain mitotic components. Similar approaches can be used to altermeiosis.

The compounds of the invention can be used to inhibit mitotic spindleformation. Such inhibition may take the form of lessening a mitotickinesin's organization of microtubules into bipolar structures,increasing or decreasing spindle pole separation, and/or inducingmitotic spindle dysfunction. In particular, the compounds of theinvention are useful to bind to and/or inhibit the activity of a mitotickinesin, KSP, especially human KSP, although KSP kinesins from otherorganisms may also be used. Also included within the definition of theterm “KSP” for these purposes are variants and/or fragments of KSP. See,U.S. Pat. No. 6,437,115. While other mitotic kinesins may be used in thepresent invention, the compounds of the invention have been shown tohave specificity for KSP. Contacting a compound of the invention with aKSP kinesin, particularly human KSP kinesin, can lead to diminishedKSP-mediated ATP hydrolysis activity and/or diminished KSP-mediatedmitotic spindle formation activity. Meiotic spindles can be similarlydisrupted.

In another embodiment, the compounds of the invention can be used tomodulate one or more other human mitotic kinesins, in addition toinhibiting KSP, including: HSET (see, U.S. Pat. No. 6,361,993); MCAK(see, U.S. Pat. No. 6,331,424); CENP-E (see, PCT Publication No. WO99/13061); Kif4 (see, U.S. Pat. No. 6,440,684); MKLP1 (see, U.S. Pat.No. 6,448,025); Kif15 (see, U.S. Pat. No. 6,355,466); Kid (see, U.S.Pat. No. 6,387,644); Mpp1, CMKrp, Kinl-3 (see, U.S. Pat. No. 6,461,855);Kip3a (see, PCT Publication No. WO 01/96593); Kip3d (see, U.S. Pat. No.6,492,151); and RabK6.

Therapeutic uses facilitated by the mitotic kinesin-inhibitory activityof the compounds of the present invention include the treatment ofdisorders associated with cell proliferation.

Particular disease states that can be treated by the methods,pharmaceutical formulations, and compounds provided herein include, butare not limited to, cancer (further discussed below), autoimmunedisease, arthritis, graft rejection, inflammatory bowel disease,proliferation induced after medical procedures, including, but notlimited to, surgery, angioplasty, and the like. In one embodiment, theinvention includes application to cells or individuals afflicted orimpending afflication with any one of these disorders or states.

The compounds, pharmaceutical formulations and methods provided hereinare particularly deemed useful for the treatment of cancer includingsolid tumors such as skin, breast, brain, cervical carcinomas,testicular carcinomas, etc. More particularly, cancers that can betreated include, but are not limited to:

-   -   Cardiac: sarcoma (angiosarcoma, fibrosarcoma, rhabdomyosarcoma,        liposarcoma), myxoma, rhabdomyoma, fibroma, lipoma and teratoma;    -   Lung: bronchogenic carcinoma (squamous cell, undifferentiated        small cell, undifferentiated large cell, adenocarcinoma),        alveolar (bronchiolar) carcinoma, bronchial adenoma, sarcoma,        lymphoma, chondromatous hamartoma, mesothelioma;    -   Gastrointestinal: esophagus (squamous cell carcinoma,        adenocarcinoma, leiomyosarcoma, lymphoma), stomach (carcinoma,        lymphoma, leiomyosarcoma), pancreas (ductal adenocarcinoma,        insulinoma, glucagonoma, gastrinoma, carcinoid tumors, vipoma),        small bowel (adenocarcinoma, lymphoma, carcinoid tumors,        Karposi's sarcoma, leiomyoma, hemangioma, lipoma, neurofibroma,        fibroma), large bowel (adenocarcinoma, tubular adenoma, villous        adenoma, hamartoma, leiomyoma);    -   Genitourinary tract: kidney (adenocarcinoma, Wilm's tumor        [nephroblastoma], lymphoma, leukemia), bladder and urethra        (squamous cell carcinoma, transitional cell carcinoma,        adenocarcinoma), prostate (adenocarcinoma, sarcoma), testis        (seminoma, teratoma, embryonal carcinoma, teratocarcinoma,        choriocarcinoma, sarcoma, interstitial cell carcinoma, fibroma,        fibroadenoma, adenomatoid tumors, lipoma);    -   Liver: hepatoma (hepatocellular carcinoma), cholangiocarcinoma,        hepatoblastoma, angiosarcoma, hepatocellular adenoma,        hemangioma;    -   Bone: osteogenic sarcoma (osteosarcoma), fibrosarcoma, malignant        fibrous histiocytoma, chondrosarcoma, Ewing's sarcoma, malignant        lymphoma (reticulum cell sarcoma), multiple myeloma, malignant        giant cell tumor chordoma, osteochronfroma (osteocartilaginous        exostoses), benign chondroma, chondroblastoma,        chondromyxofibroma, osteoid osteoma and giant cell tumors;    -   Nervous system: skull (osteoma, hemangioma, granuloma, xanthoma,        osteitis deformans), meninges (meningioma, meningiosarcoma,        gliomatosis), brain (astrocytoma, medulloblastoma, glioma,        ependymoma, germinoma [pinealoma], glioblastoma multiform,        oligodendroglioma, schwannoma, retinoblastoma, congenital        tumors), spinal cord neurofibroma, meningioma, glioma, sarcoma);    -   Gynecological: uterus (endometrial carcinoma), cervix (cervical        carcinoma, pre-tumor cervical dysplasia), ovaries (ovarian        carcinoma [serous cystadenocarcinoma, mucinous        cystadenocarcinoma, unclassified carcinoma], granulosa-thecal        cell tumors, Sertoli-Leydig cell tumors, dysgerminoma, malignant        teratoma), vulva (squamous cell carcinoma, intraepithelial        carcinoma, adenocarcinoma, fibrosarcoma, melanoma), vagina        (clear cell carcinoma, squamous cell carcinoma, botryoid sarcoma        (embryonal rhabdomyosarcoma], fallopian tubes (carcinoma);    -   Hematologic: blood (myeloid leukemia [acute and chronic], acute        lymphoblastic leukemia, chronic lymphocytic leukemia,        myeloproliferative diseases, multiple myeloma, myelodysplastic        syndrome), Hodgkin's disease, non-Hodgkin's lymphoma [malignant        lymphoma];    -   Skin: malignant melanoma, basal cell carcinoma, squamous cell        carcinoma, Karposi's sarcoma, moles dysplastic nevi, lipoma,        angioma, dermatofibroma, keloids, psoriasis; and    -   Adrenal glands: neuroblastoma.        As used herein, treatment of cancer includes treatment of        cancerous cells, induding cells afflicted by any one of the        above-identified conditions.

Another useful aspect of the invention is a kit having a compound, saltor solvate of Formula I or II and a package insert or other labelingincluding directions treating a cellular proliferative disease byadministering an effective amount of the compound, salt or solvate. Thecompound, salt or solvate of Formula I or II in the kits of theinvention is particularly provided as one or more doses for a course oftreatment for a cellular proliferative disease, each dose being apharmaceutical formulation including a pharmaceutically acceptedexcipient and a compound, salt or solvate of Formula I or II.

Testing

To assay activity, generally, either KSP or a compound according to theinvention is non-diffusably bound to an insoluble support havingisolated sample receiving areas. The insoluble support can be made ofany material to which the compounds can be bound, is readily separatedfrom soluble material, and is otherwise compatible with the overallmethod of screening. The surface of such supports can be solid or porousand of any convenient shape. Examples of suitable insoluble supportsinclude microliter plates, arrays, membranes and beads. These aretypically made of glass, plastic (e.g., polystyrene), polysaccharides,nylon or nitrocellulose, Teflon™, etc. Microtiter plates and arrays areespecially convenient because a large number of assays can be carriedout simultaneously, using small amounts of reagents and samples. Theparticular manner of binding of the compound is not crucial so long asit is compatible with the reagents and overall methods of the invention,maintains the activity of the compound and is nondiffusable. Particularmethods of binding include the use of antibodies (which do notsterically block either the ligand binding site or activation sequencewhen the protein is bound to the support), direct binding to “sticky” orionic supports, chemical crosslinking, the synthesis of the protein oragent on the surface, etc. Following binding of the protein or agent,excess unbound material is removed by washing. The sample receivingareas can then be blocked through incubation with bovine serum albumin(BSA), casein or other innocuous protein or other moiety.

The compounds of the invention can be used on their own to modulate theactivity of a mitotic kinesin, particularly KSP. In this embodiment, acompound of the invention is combined with KSP and the activity of KSPis assayed. Measurable kinesin activities include the ability to affectATP hydrolysis; microtubule binding; gliding andpolymerization/depolymerization (effects on microtubule dynamics);binding to other proteins of the spindle; binding to proteins involvedin cell-cycle control; serving as a substrate to other enzymes, such askinases or proteases; and specific kinesin cellular activities such asspindle pole separation.

Methods of performing motility assays are well known to those of skillin the art. [See e.g., Hall, et al. (1996), Biophys. J., 71: 3467-3476,Turner et al., 1996, AnaL Biochem. 242 (1):20-5; Gittes et al., 1996,Biophys. J. 70(I): 418-29; Shirakawa et al., 1995, J. Exp. BioL 198:1809-15; Winkelmann et al., 1995, Biophys. J. 68: 2444-53; Winkelmann etal., 1995, Biophys. J. 68: 72S.]

Methods known in the art for determining ATPase hydrolysis activity alsocan be used.

Solution based assays are particularly suitable (see, U.S. Pat. No.6,410,254); alternatively, conventional methods are used. For example,P_(i) release from kinesin can be quantified. In one embodiment, theATPase hydrolysis activity assay utilizes 0.3 M PCA (perchloric acid)and malachite green reagent (8.27 mM sodium molybdate II, 0.33 mMmalachite green oxalate, and 0.8 mM Triton X-1 00). To perform theassay, 10 μL of reaction is quenched in 90 μL of cold 0.3 M PCA.Phosphate standards are used so data can be converted to mM inorganicphosphate released. When all reactions and standards have been quenchedin PCA, 100 μL of malachite green reagent is added to the relevant wellsin e.g., a microtiter plate. The mixture is developed for 10-15 minutesand the plate is read at an absorbance of 650 nm.

When phosphate standards are used, absorbance readings can be convertedto mM P_(i) and plotted over time. Additionally, ATPase assays known inthe art include the luciferase assay.

ATPase activity of kinesin motor domains also can be used to monitor theeffects of modulating agents. In one embodiment ATPase assays of kinesinare performed in the absence of microtubules. In another embodiment, theATPase assays are performed in the presence of microtubules. Differenttypes of modulating agents can be detected in the above assays. In oneparticular embodiment, the effect of a modulating agent is independentof the concentration of microtubules and ATP. In another embodiment, theeffect of the agents on kinesin ATPase can be decreased by increasingthe concentrations of ATP, microtubules or both. In yet anotherembodiment, the effect of the modulating agent is increased byincreasing concentrations of ATP, microtubules or both.

Agents that modulate the biochemical activity of KSP in vitro may thenbe screened in vivo. Methods for testing such agents in vivo includeassays of cell cycle distribution, cell viability, or the presence,morphology, activity, distribution or amount of mitotic spindles.Methods for monitoring cell cycle distribution of a cell population, forexample, by flow cytometry, are well known to those skilled in the art,as are methods for determining cell viability. See, for example, WO01/31335, entitled “Methods of Screening for Modulators of CellProliferation and Methods of Diagnosing Cell Proliferation States.”

In addition to the assays described above, microscopic methods formonitoring spindle formation and malformation are well known to those ofskill in the art (see, e.g., Whitehead and Rattner (1998), J. Cell Sci.111:2551-61; Galgio et al, (1996) J. Cell Biol., 135:399-414).

The compounds of the invention inhibit KSP kinesin. One measure ofinhibition, IC₅₀, is defined as the concentration of the compound atwhich the activity of KSP is decreased by fifty percent. Particularlysuitable compounds have IC₅₀'s of less than about 1 mM, with moreparticularly suitable compounds having IC₅₀'s of less than about 100 μM.IC₅₀'s of less than about 10 nM can be attained by certain compounds ofthe invention, and the pharmaceutically acceptable salts and solvatesthereof, it being appreciated that a smaller IC₅₀ is generallyconsidered advantageous. Measurement of IC₅₀ is done using an ATPaseassay.

Another measure of inhibition is K_(i). For compounds with IC₅₀'s lessthan 1 μM, the K_(i) or K_(d) is defined as the dissociation rateconstant for the interaction of the test compound with KSP. Particularlysuitable compounds have K_(i)'s of less than about 100 μM, moreparticularly suitable compounds having K_(i)'s of less than about 10 μM.Ki's of less than about 10 nM can be attained by certain compounds ofthe invention, and the pharmaceutically acceptable salts and solvatesthereof, it being appreciated that a smaller K_(i) is generallyconsidered advantageous. The K_(i) for a compound is determined from theIC₅₀ based on three assumptions. First, only one compound molecule bindsto the enzyme and there is no cooperativity. Second, the concentrationsof active enzyme and the compound tested are known (i.e., there are nosignificant amounts of impurities or inactive forms in thepreparations). Third, the enzymatic rate of the enzyme-inhibitor complexis zero. The rate (i.e., compound concentration) data are fitted to theequation:$V = {V_{\max}{E_{0}\lbrack {I - \frac{( {E_{0} + I_{0} + {Kd}} ) - \sqrt{( {E_{0} + I_{0} + {Kd}} )^{2} - {4\quad E_{0}I_{0}}}}{2E_{0}}} \rbrack}}$Where V is the observed rate, V_(max) is the rate of the free enzyme, I₀is the inhibitor concentration, E₀ is the enzyme concentration, andK_(d) is the dissociation constant of the enzyme-inhibitor complex.

Another measure of inhibition is GI₅₀, defined as the concentration ofthe compound that results in a decrease in the rate of cell growth byfifty percent. Anti-proliferative compounds that have been successfullyapplied in the clinic to treatment of cancer (cancer chemotherapeutics)have GI₅₀'s that vary greatly. For example, in A549 cells, paclitaxelGI₅₀ is 4 nM, doxorubicin is 63 nM, 5-fluorouracil is 1 μM, andhydroxyurea is 500 μM (data provided by National Cancer Institute,Developmental Therapeutic Program, http://dtp.nci.nih.gov/). Therefore,compounds that inhibit cellular proliferation at virtually anyconcentration may be useful. Particularly suitable compounds have GI₅₀'sof less than about 1 mM, with more particularly suitable compoundshaving a GI₅₀ of less than about 10 μM. GI₅₀'s of less than about 10 nMcan be attained by certain compounds of the invention, and thepharmaceutically acceptable salts and solvates thereof, it beingappreciated that a smaller GI₅₀ is generally considered advantageous.Measurement of GI₅₀ is done using a cell proliferation assay.

Testing for growth inhibition using cell lines (such as MCF-7/ADR-RESand HCT1 5) that express P-glycoprotein (also known as Multi-drugResistance, or MDR⁺), which conveys resistance to other chemotherapeuticdrugs, such as pacilitaxel, can identify anti-mitotic agents thatinhibit cell proliferation and are not subject to resistance byoverexpression of MDR⁺ by drug-resistant tumor lines.

In vitro potency of small molecule inhibitors is determined by assayinghuman ovarian cancer cells (SKOV3) for viability following a 72-hourexposure to a 9-point dilution series of compound. Cell viability isdetermined by measuring the absorbance of formazon, a product formed bythe bioreduction of MTS/PMS, a commercially available reagent. Eachpoint on the dose-response curve is calculated as a percent of untreatedcontrol cells at 72 hours minus background absorption (complete cellkill).

To employ the compounds of the invention in a method of screening forcompounds that bind to KSP kinesin, the KSP is bound to a support, and acompound or composition of the invention is added to the assay.Alternatively, a composition of a compound of the invention bound to asolid support can be made, and KSP added to the assay. Classes ofcompounds among which novel binding agents may be sought includespecific antibodies, non-natural binding agents identified in screens ofchemical libraries, peptide analogs, etc. Of particular interest arescreening assays for candidate agents that have a low toxicity for humancells. A wide variety of assays may be used for this purpose, includinglabeled in vitro protein-protein binding assays, electrophoreticmobility shift assays, immunoassays for protein binding, functionalassays (phosphorylation assays, etc.) and the like.

The determination of the binding of the mitotic agent to KSP may be donein a number of ways. In a particular embodiment, the compound of theinvention is labeled, for example, with a fluorescent or radioactivemoiety and binding determined directly. For example, this may be done byattaching all or a portion of KSP to a solid support, adding a labeledcompound (for example a compound of the invention in which at least oneatom has been replaced by a detectable isotope), washing off excessreagent, and determining whether the amount of the label is that presenton the solid support. Various blocking and washing steps may be utilizedas is known in the art.

By “labeled” herein is meant that the compound is either directly orindirectly labeled with a label which provides a detectable signal,e.g., radioisotope, fluorescent tag, enzyme, antibodies, particles suchas magnetic particles, chemiluminescent tag, or specific bindingmolecules, etc. Specific binding molecules include pairs, such as biotinand streptavidin, digoxin and antidigoxin etc. For the specific bindingmembers, the complementary member would normally be labeled with amolecule which provides for detection, in accordance with knownprocedures, as outlined above. The label can directly or indirectlyprovide a detectable signal.

In some embodiments, only one of the components is labeled. For example,the kinesin proteins may be labeled at tyrosine positions using ¹²⁵I, orwith fluorophores. Alternatively, more than one component may be labeledwith different labels; using ¹²⁵I for the proteins, for example, and afluorophor for the anti-mitotic agents.

The compounds of the invention may also be used as competitors to screenfor additional drug candidates. “Candidate agent” or “drug candidate” orgrammatical equivalents as used herein describe any molecule, e.g.,protein, oligopeptide, small organic molecule, polysaccharide,polynucleotide, etc., to be tested for bioactivity. They may be capableof directly or indirectly altering the cellular proliferation phenotypeor the expression of a cellular proliferation sequence, including bothnucleic acid sequences and protein sequences. In other cases, alterationof cellular proliferation protein binding and/or activity is screened.Screens of this sort may be performed either in the presence or absenceof microtubules. In the case where protein binding or activity isscreened, particular embodiments exclude molecules already known to bindto that protein, for example, polymer structures such as microtubules,and energy sources such as ATP. Particular embodiments of assays hereininclude candidate agents that do not bind the cellular proliferationprotein in its endogenous native state termed herein as “exogenous”agents. In another particular embodiment, exogenous agents furtherexclude antibodies to KSP.

Candidate agents can encompass numerous chemical classes, thoughtypically they are organic molecules, particularly small organiccompounds having a molecular weight of more than 100 and less than about2,500 daltons. Candidate agents comprise functional groups necessary forstructural interaction with proteins, particularly hydrogen bonding andlipophilic binding, and typically include at least an amine, carbonyl,hydroxyl, ether, or carboxyl group, especially at least two of thefunctional chemical groups. The candidate agents often comprise cyclicalcarbon or heterocyclic structures and/or aromatic or polyaromaticstructures substituted with one or more of the above functional groups.Candidate agents are also found among biomolecules including pepfides,saccharides, fatty acids, steroids, purines, pyrimidines, derivatives,structural analogs or combinations thereof.

Candidate agents are obtained from a wide variety of sources includinglibraries of synthetic or natural compounds. For example, numerous meansare available for random and directed synthesis of a wide variety oforganic compounds and biomolecules, including expression of randomizedoligonucleotides. Alternatively, libraries of natural compounds in theform of bacterial, fungal, plant and animal extracts are available orreadily produced. Additionally, natural or synthetically producedlibraries and compounds are readily modified through conventionalchemical, physical and biochemical means. Known pharmacological agentsmay be subjected to directed or random chemical modifications, such asacylation, alkylation, esterification, amidification to producestructural analogs.

Competitive screening assays can be done by combining KSP and a drugcandidate in a first sample. A second sample may be made combining acompound of the invention, KSP and a drug candidate. This may beperformed in either the presence or absence of microtubules. The bindingof the drug candidate is determined for both samples, and a change ordifference in binding between the two samples indicates the presence ofan agent capable of binding to KSP and potentially modulating itsactivity. That is, if the binding of the drug candidate is different inthe second sample relative to the first sample, the drug candidate iscapable of binding to KSP.

In a particularly suitable embodiment, the binding of the candidateagent is determined through the use of competitive binding assays. Inthis embodiment, the competitor is a binding moiety known to bind toKSP, such as an antibody, peptide, binding partner, ligand, etc. Undercertain circumstances, there may be competitive binding as between thecandidate agent and the binding moiety, with the binding moietydisplacing the candidate agent.

In one embodiment, the candidate agent is labeled. Either the candidateagent, or the competitor, or both, is added first to KSP for a timesufficient to allow binding, if present. Incubations can be performed atany temperature that facilitates optimal activity, typically between 4and 40° C. Incubation periods are selected for optimum activity, but mayalso be optimized to facilitate rapid high throughput screening.Typically between 0.1 and 1 hour will be sufficient. Excess reagent isgenerally removed or washed away. The second component is then added,and the presence or absence of the labeled component is followed, toindicate binding.

In a particularly suitable embodiment, the competitor is added first,followed by the candidate agent. Displacement of the competitor is anindication the candidate agent is binding to KSP and thus is capable ofbinding to, and potentially modulating, the activity of KSP. In thisembodiment, either component can be labeled. Thus, for example, if thecompetitor is labeled, the presence of label in the wash solutionindicates displacement by the agent. Alternatively, if the candidateagent is labeled, the presence of the label on the support indicatesdisplacement.

In an alternative embodiment, the candidate agent is added first, withincubation and washing, followed by the competitor. The absence ofbinding by the competitor may indicate the candidate agent is bound toKSP with a higher affinity. Thus, if the candidate agent is labeled, thepresence of the label on the support, coupled with a lack of competitorbinding, may indicate the candidate agent is capable of binding to KSP.

It may be of value to identify the binding site of KSP. This can be donein a variety of ways. In one embodiment, once KSP has been identified asbinding to the compound, KSP is fragmented or modified and the assaysrepeated to identify the necessary components for binding.

Modulation is tested by screening for candidate agents capable ofmodulating the activity of KSP comprising the steps of combining acandidate agent with KSP, as above, and determining an alteration in thebiological activity of KSP. Thus, in this embodiment, the candidateagent should both bind to KSP (although this may not be necessary), andalter its biological or biochemical activity as defined herein. Themethods include both in vitro screening methods and in vivo screening ofcells for alterations in cell cycle distribution, cell viability, or forthe presence, morpohology, activity, distribution, or amount of mitoticspindles, as are generally outlined above.

Alternatively, differential screening may be used to identify drugcandidates that bind to the native KSP, but cannot bind to modified KSP.

Positive controls and negative controls may be used in the assays.Preferably all control and test samples are performed in at leasttriplicate to obtain statistically significant results. Incubation ofall samples is for a time sufficient for the binding of the agent to theprotein. Following incubation, all samples are washed free ofnon-specifically bound material and the amount of bound, generallylabeled agent determined. For example, where a radiolabel is employed,the samples may be counted in a scintillation counter to determine theamount of bound compound.

A variety of other reagents can be included in the screening assays.These include reagents like salts, neutral proteins, e.g., albumin,detergents, etc which may be used to facilitate optimal protein-proteinbinding and/or reduce non-specific or background interactions. Alsoreagents that otherwise improve the efficiency of the assay, such asprotease inhibitors, nuclease inhibitors, anti-microbial agents, etc.,may be used. The mixture of components may be added in any order thatprovides for the requisite binding.

Formulation and Administration

The compounds, pharmaceutically acceptable salts and solvates of FormulaI and II are administered at a therapeutically effective dosage, e.g., adosage sufficient to provide treatment for the disease states previouslydescribed. Human dosage levels are typically determined by escalatingdose ranging studies conducted in accordance with current Good ClinicalPractice, FDA and local guidelines. The amount of active compoundadministered will, of course, be dependent on the subject and diseasestate being treated, the severity of the affliction, the manner andschedule of administration and the judgment of the prescribingphysician.

The administration of the compounds and pharmaceutical formulations ofthe present invention can be done in a variety of ways, including, butnot limited to, orally, subcutaneously, intravenously, intranasally,transdermally, intraperitoneally, intramuscularly, intrapulmonary,vaginally, rectally, or intraoculariy. In some instances, for example,in the treatment of wounds and inflammation, the compound or compositionmay be directly applied as a solution or spray.

Pharmaceutical formulations include a compound of Formula I or II or apharmaceutically acceptable salt or solvate thereof, and one or morepharmaceutically acceptable excipients. As is known in the art,pharmaceutical excipients are secondary ingredients that function toenable or enhance the delivery of a drug or medicine in a variety ofdosage forms (e.g.: oral forms such as tablets, capsules, and liquids;topical forms such as dermal, opthalmic, and otic forms; suppositories;injectables; respiratory forms and the like). Pharmaceutical excipientsinclude inert or inactive ingredients, synergists or chemicals thatsubstantively contribute to the medicinal effects of the activeingredient. For example, pharmaceutical excipients may function toimprove flow characteristics, product uniformity, stability, taste, orappearance, to ease handling and administration of dose, for convenienceof use, or to control bioavailability. While pharmaceutical excipientsare commonly described as being inert or inactive, it is appreciated inthe art that there is a relationship between the properties of thepharmaceutical excipients and the dosage forms containing them.

Pharmaceutical excipients suitable for use as carriers or diluents arewell known in the art, and may be used in a variety of formulations.See, e.g., Remington's Pharmaceutical Sciences, 18th Edition, A. R.Gennaro, Editor, Mack Publishing Company (1990); Remington: The Scienceand Practice of Pharmacy, 20th Edition, A. R. Gennaro, Editor,Lippincott Williams & Wilkins (2000); Handbook of PharmaceuticalExcipients, 3rd Edition, A. H. Kibbe, Editor, American PharmaceuticalAssociation, and Pharmaceutical Press (2000); and Handbook ofPharmaceutical Additives, compiled by Michael and Irene Ash, Gower(1995). The concentration of a therapelitically active agent in aformulation can vary widely, from about 0.1 to 99.9 wt. %, depending onthe nature of the formulation.

Oral solid dosage forms such as tablets will typically comprise one ormore pharmaceutical excipients, which may for example help impartsatisfactory processing and compression characteristics, or provideadditional desirable physical characteristics to the tablet. Suchpharmaceutical excipients may be selected from diluents, binders,glidants, lubricants, disintegrants, colorants, flavorants, sweeteningagents, polymers, waxes or other solubility-modulating materials.

Dosage forms for parenteral administration will generally comprisefluids, particularly intravenous fluids, i.e., sterile solutions ofsimple chemicals such as sugars, amino acids or electrolytes, which canbe easily carried by the circulatory system and assimilated. Such fluidsare typically prepared with water for injection USP. Fluids usedcommonly for intravenous (IV) use are disclosed in Remington, TheScience and Practice of Pharmacy [full citation previously provided],and include:

-   -   alcohol, e.g., 5% alcohol (e.g., in dextrose and water (“D/W”)        or D/W in normal saline solution (“NSS”), including in 5%        dextrose and water (“D5/W”), or D5/W in NSS);    -   synthetic amino acid such as Aminosyn, FreAmine, Travasol, e.g.,        3.5 or 7; 8.5; 3.5, 5.5 or 8.5 % respectively;    -   ammonium chloride e.g., 2.14%;    -   dextran 40, in NSS e.g., 10% or in D5/W e.g., 10%;    -   dextran 70, in NSS e.g., 6% or in D5/W e.g., 6%;    -   dextrose (glucose, D5/W) e.g., 2.5-50%;    -   dextrose and sodium chloride e.g., 5-20% dextrose and 0.22-0.9%        NaCl;    -   lactated Ringer's (Hartmann's) e.g., NaCl 0.6%, KCl 0.03%, CaCl₂        0.02%;    -   lactate 0.3%;    -   mannitol e.g., 5%, optionally in combination with dextrose e.g.,        10% or NaCl e.g., 15 or 20%;    -   multiple electrolyte solutions with varying combinations of        electrolytes, dextrose, fructose, invert sugar Ringer's e.g.,        NaCl 0.86%, KCl 0.03%, CaCl₂ 0.033%;    -   sodium bicarbonate e.g., 5%;    -   sodium chloride e.g., 0.45, 0.9, 3, or 5%;    -   sodium lactate e.g., ⅙ M; and    -   sterile water for injection        The pH of such IV fluids may vary, and will typically be from        3.5 to 8 as known in the art.

The compounds, pharmaceutically acceptable salts and solvates of theinvention can be administered alone or in combination with othertreatments, i.e., radiation, or other therapeutic agents, such as thetaxane class of agents that appear to act on microtubule formation orthe camptothecin class of topoisomerase I inhibitors. When so-used,other therapeutic agents can be administered before, concurrently(whether in separate dosage forms or in a combined dosage form), orafter administration of an active agent of the present invention.

The following examples serve to more fully describe the manner of usingthe above-described invention, as well as to set forth the best modescontemplated for carrying out various aspects of the invention. It isunderstood that these examples in no way serve to limit the true scopeof this invention, but rather are presented for illustrative purposes.

EXAMPLES Example 13-Benzyl-7-chloro-1-pyrrolidine-2-yl-2,3-dihydro-1H-quinazoline-4-one

1A. Formula 103 where T is a covalent bond: W, X, Y and Z are —C═: U′—V′is —N(R^(6′))—CR^(e)R^(f)—: R¹, R², R⁴, and R^(a) to R^(f) are H: R³ ischloro; and R^(6′) is Boc A solution of pyrrolidine-1,2-dicarboxylicacid 1-tert-butyl ester (6.0 g, 28 mmol), 2-amino-4-chloro-benzoic acidmethyl ester (4.6 g, 25 mmol), PyBroP (14.4 g, 31 mmol),diisopropylethylamine (6 mL) in pyridine (60 mL) was stirred overnightat room temperature, after which HPLC/MS showed complete conversion ofstarting material. The solvents were removed under vacuum and theresidue partitioned between ethyl acetate (100 mL) and water (50 mL).The organic layer was washed with 1M HCl (50 mL) and saturated NaHCO₃(50 mL), dried over MgSO₄, filtered, and concentrated under vacuum.Silica gel chromatography using 30% ethyl acetate/hexanes as eluent gave9.3 g (87% yield) of the desired product of Formula 103,2-(5-chloro-2-methoxycarbonyl-phenylcarbamoyl)-pyrrolidine-1-carboxylicacid tert-butyl ester, as a yellow foam. LRMS (MH⁺) m/z 383.

1B. Formula 104 where T is a covalent bond; W, X, Y and Z are —C═; U′—V′is —N(R^(6′))—CR^(e)R^(f)—: R¹, R², R⁴, and R^(a) to R^(f) are H; R³ ischloro; and R^(6′) is Boc; A solution of the pure compound of Formula103 (9.3 g, 24 mmol) dissolved in 125 mL of ethanol was treated withNaOH solution (3.1 g in 16 mL of H₂O). The mixture was stirred at roomtemperature overnight and then concentrated under vacuum. The residuewas treated with a solution of saturated NaCl (200 mL) and concentratedphosphoric acid (5 mL) for a few minutes and then extracted withdichloromethane (2×200 mL). The organic fractions were combined, driedover MgSO₄, filtered and concentrated give 8.7 g (97% yield) of theclean desired product of Formula 104,2-(5-chloro-2-carboxy-phenylcarbamoyl)-pyrrolidine-1-carboxylic acidtert-butyl ester, as an orange foam. LRMS (M) m/z 368.

1C. Formula 105 where T is a covalent bond; W, X, Y and Z are —C═; U′—V′is —N(R⁶)—CR^(e)R^(f)—; R¹, R², R⁴, and R^(a) to R^(f) are H; R³ ischloro: R⁵ is benzyl. and R^(6′) is Boc:

To a solution of the compound of Formula 104 (8.7 9, 24 mmol) indichloromethane (250 mL) was added EDC (14.85 g, 77 mmol), anddiisopropylethylamine (14.1 mL, 81 mmol). After stirring at roomtemperature for 1.5 hours, benzylamine (8.4 mL, 77 mmol) was added andthe reaction stirred for an additional 48 hours. The mixture was washedwith water (2×50 mL), 10% phosphoric acid (2×50 mL), and saturatedNaHCO₃ (50 mL). The organic phase was dried over MgSO₄, filtered andconcentrated under vacuum to give a mixture of 9.4 g (88% yield) of thedesired product of Formula 105,2-(2-benzylcarbamoyl-5-chloro-phenylcarbamoyl)-pyrrolidine-1-carboxylicacid tert-butyl ester, as a light yellow foam, together with a smallamount of2-(3-benzyl-7-chloro-4-oxo-3,4-dihydro-2Hquinzoline-1-yl)-pyrrolidine-1-carboxylicacid tert-butyl ester (Formula 106), which was taken on without furtherpurification. LRMS (MH⁺) m/z 458.

1D. Formula 106 where T is a covalent bond: W, X, Y and Z are —C═: U′—V′is —N(R^(6′))—CR^(e)R^(f)—: R¹, R², R⁴, and R^(a) to R^(f) are H; R³ ischloro; R⁵ is benzyl, and R^(6′) is Boc;

The mixture of Formulae 105 and 106 (5.4 g, 12 mmol) was stirred inethylene glycol (32 mL) with K₂CO₃ (27 g) at 120° C. overnight, afterwhich the solution was allowed to cool to room temperature and wasextracted with dichloromethane (3×200 mL). The organic fractions werecombined, dried over MgSO₄, filtered and concentrated under vacuum. Theresulting residue was purified by silica gel chromatography using 20%ethyl acetate/hexanes as eluent to give 1.4 g (27% yield) of the puredesired product of Formula 106,2-(3-benzyl-7-chloro-4-oxo-3,4-dihydro-2Hquinzoline-1-yl)-pyrrolidine-1-carboxylicacid tert-butyl ester. LRMS (MH⁺) m/z 439.

1E. Formula I where U—V is —N(R⁶)—CR^(e)R^(f)—; R¹, R², R⁴, R⁶ and R^(a)to R^(f) are H; R³ is chloro; and R⁵ is benzyl: The pure compound ofFormula 106 (446 mg, 1.0 mmol) was treated with 20 mL of a 1:1 mixtureof TFA/dichloromethane for 1 hour at room temperature. The solution wasconcentrated under vacuum and partitioned between dichloromethane (20mL) and saturated NaHCO₃ (10 mL). The aqueous layer was extracted withdichloromethane (10 mL) and the combined organic layers were dried overK₂CO₃, filtered and concentrated to give 344 mg (100% yield) of thedesired product of Formula I,3-benzyl-7-chloro-1-pyrrolidine-2-yl-2,3-dihydro-1H-quinazoline-4-one,which was taken on without further purification. LRMS (MH⁺) m/z 340.

Example 23-Benzyl-7-chloro-2-]1-(4-methyl-benzoyl)-pyrrolidin-2-yl]-3H-quinazolin-4-one

Formula I where U—V is —N(R⁶)—CR^(e)R^(f)—; R¹, R², R⁴, and R^(a) toR^(f) are H; R³ is chloro; R⁵ is benzyl: and R⁶ is p-methyl-benzoyl: Toa solution of crude3-benzyl-7-chloro-1-(pyrrolidine-2-carbonyl)-2,3-dihydro-1H-quinazoline-4-one(250 mg, 0.73 mmol) in dichloromethane (4.5 mL) was added p-toluoylchloride (0.29 mL, 2.2 mmol) and diisopropylethylamine (0.77 mL, 4.4mmol). The mixture was stirred for 1 hour and then partitioned betweensaturated NaHCO₃ (10 mL) and ethyl acetate (20 mL). The organic phasewas washed with saturated NaHCO₃ (3×4 mL) and saturated NaCl (2×4 mL),dried over Na₂SO₄, filtered and concentrated under vacuum. The cruderesidue was purified by preparative reverse phase C18 HPLC (10-98%acetonitrile/water) to give 223 mg (66% yield) of the pure desiredproduct of Formula I,3-benzyl-7-chloro-2-[1-(4-methyl-benzoyl)-pyrrolidin-2-yl]-3H-quinazolin-4-one.LRMS (MH⁺) m/z 459.

Example 33-Benzyl-7-chloro-2-[1-(4-methyl-benzyl)-pyrrolidin-2-yl]-3H-quinazolin-4-one

Formula I where U—V is —N(R⁶)—CR^(e)R^(f)—; R¹, R², R⁴, and R^(a) toR^(f) are H; R³ is chloro; R⁵ is benzyl: and R⁶ is p-methyl-benzyl:Crude3-benzyl-7-chloro-1-(pyrrolidine-2-carbonyl)-2,3-dihydro-1H-quinazoline-4-one(170 mg, 0.50 mmol) dissolved in dichloromethane (5 mL) was treated withp-tolualdehyde (80 μL, 0.7 mmol) and NaHB(OAc)₃ (350 mg, 1.65 mmol), andthe mixture was stirred for 40 minutes at room temperature. The reactionwas quenched with saturated NaHCO₃ (2.2 mL) and the aqueous phaseextracted with dichloromethane (25 mL). The organic layers were combinedand dried over Na₂SO₃, filtered and concentrated under vacuum. The cruderesidue was purified by silica gel chromatography using a stepwisegradient of 20% ethyl acetate/hexanes to 50% methanol/ethyl acetate aseluent to give 52.5 mg (24% yield) of desired product of Formula I,3-benzyl-7-chloro-2-[1-(4-methyl-benzyl)-pyrrolidin-2-yl]-3Hquinazolin4-one.LRMS (MH⁺) m/z 445.

Example 4 Other Compounds of Formulae I and II

4A. Formula I where U—V is —CR^(e)R^(f)—CR^(g)R^(h)—N(R⁶)—; R¹, R², R⁴,R^(b) and R^(c) to R^(h) are H; R³ is chloro; R⁵ is methyl: and R^(a) isphenethyl: and R⁶ is D-methyl-benzoyl By following the proceduredescribed in Example 1 and substituting pyrrolidine-1,2-dicarboxylicacid 1-tert-butyl ester with 3-phenethyl-piperidine-1,4-dicarboxylicacid tert-butyl ester (in Example 1A), and substituting benzylamine withmethylamine (in Example 1C) there is obtained:7-chloro-2-[3-phenethyl-piperidin-4-yl]-3-methyl-3H-quinazolin-4-one.

4B. Formula I where U—V is —CR^(e)R—CR^(g)R^(h)—N(R⁶)—; R¹, R², R⁴,R^(b) and R^(c) to R^(h) are H; R³ is chloro; R⁵ is methyl; and R^(a) isphenethyl; and R⁶ is p-methyl-benzoyl: By following the proceduredescribed in Example 2 and substituting3-benzyl-7-chloro-1-(pyrrolidine-2-carbonyl)-2,3-dihydro-1H-quinazoline-4-onewith7-chloro-2-[3-phenethyl-piperidin-4-yl]-3-methyl-3H-quinazolin-4-one,there is obtained:7-chloro-2-[1-(4-methyl-benzoyl)-3-phenethyl-piperidin-4-yl]-3-methyl-3H-quinazolin-4-one.

4C. Formula I where U—V is —CR^(e)R^(f)—CR^(g)R^(h)—N(R⁶)—; R¹, R⁴,R^(b) and R^(c) to R^(h) are H; R² and R³ are methoxy; R⁵ is methyl; andR^(a) is phenethyl; and R⁶ is benzyl: By following the proceduredescribed in Example 3, substituting3-benzyl-7-chloro-1-(pyrrolidine-2-carbonyl)-2,3-dihydro-1H-quinazoline-4-onewith6,7-dimethoxy-2-[3-phenethyl-piperidin-4-yl]-3-methyl-3H-quinazolin-4-one,and substituting p-tolualdehyde with benzaldehyde, there is obtained6,7-dimethoxy-2-[1-benzyl-3-phenethyl-piperidin4-yl]-3-methyl-3H-quinazolin-4-one.

4D. Compounds of Formula II Varying T By following the proceduredescribed in Example 1 and substituting pyrrolidine-1,2-dicarboxylicacid 1-tert-butyl ester with the following:

-   -   2-carboxymethyl-pyrrolidine-1-carboxylic acid tert-butyl ester;    -   2-(2-carboxy-acetyl)-pyrrolidine-1-carboxylic acid tert-butyl        ester; and    -   2-(2-carboxy-ethyl)-piperidine-1-carboxylic acid tert-butyl        ester,        there are obtained the following respective compounds:    -   3-benzyl-7-chloro-2-pyrrolidine-2-ylmethyl-3H-quinazoline-4-one;    -   3-benzyl-7-chloro-2-(2-oxo-2-pyrrolidine-2-yl-ethyl)-3H-quinazoline-4-one;        and    -   3-benzyl-7-chloro-2-piperidine-2-ylethyl-3H-quinazoline-4-one.

E. Compounds of Formula II Varying W, X, Y and Z By following theprocedure described in Example 1 and substituting2-amino-4-chloro-benzoic acid methyl ester with the following:

-   -   4-amino-6-chloro-nicotinic acid methyl ester;    -   3-amino-pyrazine-2-carboxylic acid;    -   3-amino-1,4-dihydro-pyridine-2-carboxylic acid;    -   2-amino-cyclopent-1-enecarboxylic acid;    -   4-amino-2,5-dihydro-furan-3-carboxylic acid; and    -   3-amino-1H-pyrrole-2-carboxylic acid,        there are obtained the following respective compounds:    -   3-benzyl-7-chloro-1-pyrrolidine-2-yl-2,3-dihydro-1H-pyrido[4,3-d]pyrimidin-4-one;    -   3-benzyl-1-pyrrolidin-2-yl-1H-pteridin-4-one;    -   3-benzyl-1-pyrrolidin-2-yl-2,3,5,6,7,8-hexahydro-1H-pyrido[3,2-d]pyrimidin-4-one;    -   3-benzyl-1-pyrrolidin-2-yl-1,2,3,5,6,7-hexahydro-cyclopentapyrimidin-4-one;    -   3-benzyl-1-pyrrolidin-2-yl-2,3,5,7-tetrahydro-1H-furo[3,4-d]pyrimidin-4-one;        and    -   3-benzyl-1-pyrrolidin-2-yl-1,2,3,5tetrahydro-pyrrolo[3,2-d]pyrimidin-4-one.

Example 5 3-Benzyl-7-chloro-2-[1-(4-methyl-benzoyl)-piperidin-2-yl]-3H-quinazolin-4-one

5A. Formula 202 where T is a covalent bond: U—V is—N(⁶)—CR^(e)R^(f)—CR^(g)R^(h)—: R^(a) to R^(h) are H; R³ is chloro: andR⁶ is p-methyl-benzoyl: To a solution of piperidine-2-carboxylic acid(3.5 g, 15 mmol) in 2M NaOH (70 mL) at 0° C., was added p-toluoylchloride (2.2 mL, 17 mmol) in 10 equal portions over 1 hour. The mixturewas then stirred an additional 1 hour at room temperature. The reactionwas cooled to 0° C. and partitioned between 1N HCl and dichloromethane.The organic layer was dried over MgSO₄, filtered and concentrated undervacuum to give 4.7 g of oil. 2.0 g of this material was recrystallizedfrom 95% ethanol (2.5 mL) to give 0.86 g of the desired pure crystallineproduct of Formula 202, 1-(4-methyl-benzoyl)-piperidine-2-carboxylicacid. LRMS (MH⁺) m/z 248.

5B. Forrmula 203 where T is a covalent bond; W, X, Y and Z are —C═; U—Vis —N(R⁶)—CR^(e)R^(f)—CR^(g)R^(h)—; R¹, R², R⁴ and R^(a) to R^(h) are H;R³ is chloro: and R⁶ is p-methyl-benzoyl:

In an ice bath at −15° C., pure compound of Formula 202 (7.2 g, 29 mmol)was combined with 2-amino-4-chloro-benzoic acid methyl ester (4.5 g, 24mmol), PyBroP (2.3 g, 4.9 mmol) and diisopropylethylamine (0.95 mL, 5.4mmol) in DMF (30 mL). The resulting mixture was stirred ovemight,allowing the temperature to rise to room temperature with the bath. Thesolution was concentrated under vacuum and the residue partitionedbetween ethyl acetate (200 mL) and water (200 mL). The organic layer waswashed with water (3×100 mL) and concentrated under vacuum. Theresulting residue was purified by silica gel chromatography using 40%ethyl acetate/hexanes as eluent to give 2.66 g (21% yield) of thedesired product of Formula 203,4-chloro-2-{[1-(4-methyl-benzoyl)-piperidine-2-carbonyl]amino}-benzoicacid methyl ester, as a crystalline solid. LRMS (MH⁺) m/z 472.

5C. Formula 205 where T is a covalent bond; W, X, Y and Z are —C═: U—Vis —N(R⁶)—CR^(e)R^(f)—CR^(g)R^(h)—; R¹, R², R⁴ and R^(a) to R^(h) are H;R^(and R) ⁶ is p-methyl-benzoyl: A mixture of the compound of Formula203 (2.66 g, 6.7 mmol), 1M NaOH (27 mL), methanol (54 mL), and dioxane(36 mL) was stirred at room temperature for 7 hours. The solvents wereevaporated and the residue portioned between dichloromethane (75 mL),saturated NaCl (50 mL), and concentrated HCl (3 mL). The layers wereseparated and the organic layer dried over MgSO₄ and concentrated undervacuum to give the corresponding compound of Formula 204,4-chloro-2-[1-(4-methyl-benzoyl)-piperidine-2-carbonyl]amino}-benzoicacid, which was carried forward without further purification. This crudeproduct was redissolved in dichloromethane and treated with EDC (3.84 g,20 mmol), and diisopropylethylamine (3.50 mL, 20 mmol). After stirring20 minutes, benzylamine (2.20 mL, 20 mmol) was added and the mixturestirred overnight at room temperature. The mixture was washed with water(2×50 mL) and 1M HCl, dried over MgSO₄, filtered and concentrated undervacuum to give 1.5 g (47% yield) of the desired product of Formula 205,1-(4-methyl-benzoyl)-piperidine-2-carboxylic acid(2-benzylcarbamoyl-5-chloro-phenyl-amide, a crude product that was foundto be 80% pure by reverse phase HPLC. The product was taken on withoutfurther purification. LRMS (MH⁺) m/z 491.

5D. Formula I where U—V is —N(R⁶)—CR^(e)R^(f)—CR^(g)R^(h)—; R¹, R², R⁴and R^(a) to R^(h) are H; R³ is chloro; R⁵ is benzyl; and R⁶ isp-methyl-benzoyl: A mixture of the crude product of Formula 205 (170 mg,0.35 mmol) and K₂CO₃ (0.8 g, 5.8 mmol) in ethylene glycol (4 mL) wasstirred at 120° C. overnight. After cooling to room temperature, thesolution was diluted with water (30 mL), and extracted withdichloromethane (30 mL) and ethyl acetate (30 mL). The organic layerswere combined and washed with water (2×5 mL) and saturated NaCl (5 mL),dried over Na₂SO₄, filtered and concentrated under vacuum to give aturbid yellow oil. The crude material was purified by reverse phase C18HPLC (10-98% acetonitrile/water) to give 19 mg (12% yield) of thedesired product of Formula I,3-benzyl-7-chloro-2-[1-(4-methyl-benzoyl)-piperidin-2-yl]-3H-quinazolin-4-one.LRMS (MH⁺) m/z 473.

Example 6 Other Compounds of Formulae I and II

6A. Formula I Varving R⁶: By following the procedure described inExample 5 and substituting p-toluoyl chloride with the following:

-   -   benzyl chloride;    -   benzoyl chloride; and    -   p-tolyl chloride,        there are obtained the following respective compounds:    -   3-benzyl-7-chloro-2-(1-benzyl-piperidin-2-yl)-3H-quinazolin-4-one;    -   3-benzyl-7-chloro-2-(1-benzoyl-piperidin-2-yl)-3H-quinazolin-4-one;        and    -   3-benzyl-7-chloro-2-[1-(4-methyl-benzyl)-piperidin-2-yl]-3H-quinazolin-4-one.

6B. Compounds of Formula II Varving T By following the proceduredescribed in Example 5 and substituting piperidine-1,2-dicarboxylic acidwith the following:

-   -   2-carboxymethyl-pyrrolidine-1-carboxylic acid;    -   2-(2-carboxy-acetyl)-pyrrolidine-1-carboxylic acid; and    -   2-(2-carboxy-ethyl)-piperidine-1-carboxylic acid,        there are obtained the following respective compounds:    -   3-benzyl-7-chloro-2-[1-(4-methyl-benzoyl)-pyrrolidine-2-ylmethyl]-3H-quinazoline-4-one;    -   3-benzyl-7-chloro-2-{2-[1-(4-methyl-benzoyl)-pyrrolidine-2-yl]-2-oxo-ethyl}-3H-quinazoline-4-one;        and    -   3-benzyl-7-chloro-2-[1-(4-methyl-benzoyl)-piperidine-2-ylethyl]-3H-quinazoline-4-one.

6C. Compounds of Formula II Varying W, X, Y and Z By following theprocedure described in Example 5 and substituting2-amino-4-chloro-benzoic acid methyl ester with the following:

-   -   4-amino-6-chloro-nicotinic acid methyl ester;    -   3-aminopyrazine-2-carboxylic acid;    -   3-amino-1,4-dihydro-pyridine-2-carboxylic acid;    -   2-amino-cyclopent-1-enecarboxylic acid;    -   4-amino-2,5-dihydro-furan-3-carboxylic acid; and    -   3-amino-1H-pyrrole-2-carboxylic acid,        there are obtained the following respective compounds:    -   3-benzyl-7-chloro-1-(4-methyl-benzoyl)pyrrolidine-2-yl-2,3-dihydro-1H-pyrido[4,3-d]pyrimidin-4-one;    -   3-benzyl-1-(4-methyl-benzoyl)-pyrrolidin-2-yl-1H-pteridin-4-one;    -   3-benzyl-1-(4-methyl-benzoyl)-pyrrolidin-2-yl-2,3,5,6,7,8-hexahydro-1H-pyrido[3,2-d]pyrimidin-4-one;    -   3-benzyl-1-(4-methyl-benzoyl)-pyrrolidin-2-yl-1,2,3,5,6,7-hexahydro-cyclopentapyrimidin-4-one;    -   3-benzyl-1-(4-methyl-benzoyl)-pyrrolidin-2-yl-2,3,5,7-tetrahydro-1H-furo[3,4-d]pyrimidin-4-one;        and    -   3-benzyl-1-(4-methyl-benzoyl)-pyrrolidin-2-yl-1,2,3,5-tetrahydro-pyrrolo[3,2-d]pyrimidin-4-one.

Example 7 Induction of Mitotic Arrest in Cell Populations Treated with aKSP Inhibitor

FACS analysis to determine cell cycle stage by measuring DNA content isperformed as follows. Skov-3 cells (human ovarian cancer) are split 1:10for plating in 10cm dishes and grown to subconfluence with RPMI 1640medium containing 5% fetal bovine serum (FBS). The cells are thentreated with either 10 nM paclitaxel, 400 nM test compound, 200 nM testcompound, or 0.25% DMSO (vehicle for compounds) for 24 hours. A wellknown anti-mitotic agent, such as placitaxel, is used as a positivecotnrol. Cells are then rinsed off the plates with PBS containing 5mMEDTA, pelleted, washed once in PBS containing 1% FCS, and then fixedovernight in 85% ethanol at 4° C. Before analysis, the cells arepelleted, washed once, and stained in a solution of 10 μg propidiumiodide and 250 μg of ribonuclease (RNAse) A per milliliter at 37° C. forhalf an hour. Flow cytometry analysis is perforned on a Becton-DickinsonFACScan, and data from 10,000 cells per sample is analyzed with Modfitsoftware.

Monopolar Spindle Formation Following Application of a Quinazolinone KSPInhibitor

To determine the nature of G2/M accumulation, human tumor cell linesSkov-3 (ovarian), HeLa (cervical), and A549 (lung) are plated in 96-wellplates at densities of 4,000 cells per well (SKOV-3 & HeLa) or 8,000cells per well (A549), allowed to adhere for 24 hours, and treated withvarious concentrations of the test compounds for 24 hours. Cells arefixed in 4% formaldehyde and stained with antitubulin antibodies(subsequently recognized using fluorescently-labeled secondary antibody)and Hoechst dye (which stains DNA). The cells can be visually inspectedto assess the effects of the test compounds. For example, microinjectionof anti-KSP antibodies causes mitotic arrest with arrested cellsdisplaying monopolar spindles.

Example 8 Inhibition of Cellular Proliferation in Tumor Cell LinesTreated with KSP Inhibitors

Cells are plated in 96-well plates at densities from 1000-2500cells/well (depending on the cell line) and allowed to adhere/grow for24 hours. They are then treated with various concentrations of testcompound for 48 hours. The time at which compounds are added isconsidered To. A tetrazolium-based assay using the reagent3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium(MTS) (U.S. Pat. No. 5,185,450) (see Promega product catalog #G3580,CellTiter 96® AQ_(ueous) One Solution Cell Proliferation Assay) is usedto determine the number of viable cells at T₀ and the number of cellsremaining after 48 hours compound exposure. The number of cellsremaining after 48 hours is compared to the number of viable cells atthe time of test compound addition, allowing for calculation of growthinhibition. The growth over 48 hours of cells in control wells treatedwith vehicle only (0.25% DMSO) is considered 100% growth and the growthof cells in wells with compounds is compared to this. Active KSPinhibitors inhibit cell proliferation in one or more human tumor celllines of the following tumor types: lung (NCI-H460, A549), breast(MDA-MB-231, MCF-7, MCF-7/ADR-RES), colon (HT29, HCT15), ovarian(SKOV-3, OVCAR-3), leukemia (HL-60(TB), K-562), central nervous system(SF-268), renal (A498), osteosarcoma (U2-OS), and cervical (HeLa), andmouse tumor line (B16, melanoma).

Calculation Of GI₅₀: A GI150 is calculated by plotting the concentrationof compound in μM vs the percentage of cell growth of cell growth intreated wells. The GI₅₀ calculated for the compounds is the estimatedconcentration at which growth is inhibited by 50% compared to control,i.e., the concentration at which:100×[(Treated₄₈ −T ₀)/(Control₄₈ −T ₀)]=50.All concentrations of compounds are tested in duplicate and controls areaveraged over 12 wells. A very similar 96-well plate layout and GI₁₅calculation scheme is used by the National Cancer Institute (see Monks,et al., J. Natl. Cancer Inst. 83:757-766 (1991)). However, the method bywhich the National Cancer Institute quantitates cell number does not useMTS, but instead employs altemative methods.

Calculation Of IC₅₀: Measurement of a compound's IC₅₀ for KSP activityuses an ATPase assay. The following solutions are used: Solution 1consists of 3 mM phosphoenolpyruvate potassium salt (Sigma P-7127), 2 mMATP (Sigma A-3377), 1 mM IDTT (Sigma D-9779), 5 μM paclitaxel (SigmaT-7402), 10 ppm antifoam 289 (Sigma A-8436), 25 mM Pipes/KOH pH 6.8(Sigma P6757), 2 mM MgCl₂ (VWR JT400301), and 1 mM EGTA (Sigma E3889).Solution 2 consists of 1 mM NADH (Sigma N8129), 0.2 mg/ml BSA (SigmaA7906), pyruvate kinase 7U/ml, L-lactate dehydrogenase 10 U/mI (SigmaP0294), 100 nM KSP motor domain, 50 μg/ml microtubules, 1 mM DTT (SigmaD9779), 5 ;M paclitaxel (Sigma T-7402), 10 ppm antifoam 289 (SigmaA-8436), 25 mM Pipes/KOH pH 6.8 (Sigma P6757), 2 mM MgCl₂ (VWRJT4003-01), and 1 mM EGTA (Sigma E3889). Serial dilutions (8-12 two-folddilutions) of the composition are made in a 96-well microtiter plate(Coming Costar 3695) using Solution 1. Following serial dilution eachwell has 50 μl of Solution 1. The reaction is started by adding 50 μl ofSolution 2 to each well. This can be done with a multichannel pipettoreither manually or with automated liquid handling devices. Themicrotiter plate is then transferred to a microplate absorbance readerand multiple absorbance readings at 340 nm are taken for each well in akinetic mode. The observed rate of change, which is proportional to theATPase rate, is then plotted as a function of the compoundconcentration. For a standard IC₅₀ determination the data acquired isfit by the following four parameter equation using a nonlinear filtingprogram (e.g., Grafit 4):$y = {\frac{Range}{1 + ( \frac{x}{{IC}_{50}} )^{S}} + {Background}}$where y is the observed rate and x the compound concentration.

Example 9 Inhibition of Cellular Viability in Tumor Cell Lines Treatedwith KSP Inhibitors

Materials and Solutions:

-   -   Cells: SKOV3, Ovarian Cancer (human).    -   Media: Phenol Red Free RPMI+5% Fetal Bovine Serum+2 mM        L-glutamine.    -   Colorimetric Agent for Determining Cell Viability: Promega MTS        tetrazolium compound.    -   Control Compound for max cell kill: Topotecan, 1 μM.

Procedure: Day 1—Cell Plating: Adherent SKOV3 cells are washed with 10mLs of PBS followed by the addition of 2 mLs of 0.25% trypsin andincubation for 5 minutes at 37° C. The cells are rinsed from the flaskusing 8 mL of media (phenol red-free RPMI+ 5% FBS) and transferred tofresh flask. Cell concentration is determined using a Coulter counterand the appropriate volume of cells to achieve 1000 cells/100 μL iscalculated. 100 μL of media cell suspension (adjusted to 1000 cells/100μL) is added to all wells of 96-well plates, followed by incubation for18 to 24 hours at 37° C., 100% humidity, and 5% CO_(2,) allowing thecells to adhere to the plates.

Procedure: Day 2—Compound Addition: To one column of the wells of anautoclaved assay block are added an initial 2.5 μL of test compound(s)at 400× the highest desired concentration. 1.25 μL of 400×(400μM)Topotecan is added to other wells (ODs from these wells are used tosubtract out for background absorbance of dead cells and vehicle). 500μL of media without DMSO are added to the wells containing testcompound, and 250 μL to the Topotecan wells. 250 μL of media +0.5% DMSOis added to all remaining wells, into which the test compound(s) areserially diluted. By row, compound-containing media is replica plated(in duplicate) from the assay block to the corresponding cell plates.The cell plates are incubated for 72 hours at 37° C., 100% humidity, and5% CO₂.

Procedure: Day 4—MTS Addition and OD Reading: The plates are removedfrom the incubator and 40 μl MTS/PMS is added to each well. Plates arethen incubated for 120 minutes at 37° C., 100% humidity, 5% CO₂,followed by reading the ODs at 490 nm after a 5 second shaking cycle ina ninety-six well spectrophotometer.

Data Analysis The normalized % of control (absorbance-background) iscalculated and an XLfit is used to generate a dose-response curve fromwhich the concentration of compound required to inhibit viability by 50%is determined.

The compounds of the present invention show activity when tested in oneor more of the methods described in Examples 7, 8 and 9.

While the present invention has been described with reference to thespecific embodiments thereof, it should be understood by those skilledin the art that various changes may be made and equivalents may besubstituted without departing from the true spirit and scope of theinvention. In addition, many modifications may be made to adapt aparticular situation, material, composition of matter, process, processstep or steps, to the objective, spirit and scope of the presentinvention. All such modifications are intended to be within the scope ofthe claims appended hereto. All patents and publications cited above arehereby incorporated by reference.

1. A compound selected from the group represented by Formula I:

where: U—V is —N(R⁶)—CR^(e)R^(f)—, —CR^(e)R^(f)—N(R⁶)—,—N(R⁶)—CR^(e)R^(f)—CR^(g)R^(h), —CR^(e)R^(f)—N(R⁶)—CR^(g)R^(h)— or—CR^(e)R^(f)—CR^(g)R^(h)—N(R⁶)—; R^(a), R^(b), R^(c), R^(d), R^(e),R^(f), R^(g) and R^(h) are independently hydrogen, alkyl, aryl, aralkyl,heteroaryl, substituted alkyl, substituted aryl, substituted aralkyl orsubstituted heteroaryl; R¹, R², R³, and R⁴ are independently hydrogen,alkyl, alkoxy, halogen, cyano or substituted alkyl; R⁵ is alkyl, aryl,aralkyl, heteroaryl, heteroaralkyl, substituted alkyl, substituted aryl,substituted aralkyl, substituted heteroaryl or substitutedheteroaralkyl; and R⁶ is hydrogen, acyl, alkyl, aryl, aralkyl,heteroaryl, substituted acyl, substituted alkyl, substituted aryl,substituted aralkyl or substituted heteroaryl; or a pharmaceuticallyacceptable salt or solvate thereof.
 2. The compound of claim 1comprising one or more of the following: R¹, R², R³ and R⁴ areindependently hydrogen, chloro, fluoro, methyl, methoxy, cyano orsubstituted lower alkyl; R⁵ is aralkyl or substituted aralkyl; R^(a) toR^(h) are independently hydrogen, lower alkyl or substituted loweralkyl; U—V is —N(R⁶)—CR^(e)R^(f)—CR^(g)R^(h),—CR^(e)R^(f)—N(R⁶)—CR^(g)R^(h)— or —CR^(e)R^(f)CR^(g)R^(h)—N(R⁶)—; R⁶ isoptionally substituted aralkyl or optionally substituted acyl; and is an(R)-enantiomer.
 3. The compound of claim 2 comprising one or more of thefollowing: R¹, R², R³ and R⁴ are independently hydrogen, chloro, fluoro,methyl, methoxy or cyano; R⁵ is benzyl or substituted benzyl; no morethan one of R^(a) to R^(h) is other than hydrogen; U—V is—N(R⁶)—CR^(e)R—CR^(g)R^(h)— or —CR^(e)R^(f)—N(R⁶)—CR^(g)R^(h)—; and R⁶is optionally substituted acyl.
 4. The compound of claim 3 comprisingone or more of the following: R¹, R², R³ and R⁴ are hydrogen, or threeof R¹, R², R³ and R⁴ are hydrogen and the fourth is halo, methoxy,methyl or cyano; R⁵ is benzyl; R^(a) to R^(h) are hydrogen; U—V is—N(R⁶)—CR^(e)R^(f)—CR^(g)R^(h)—; and R⁶ is p-methyl-benzoyl.
 5. Thecompound of claim 4 where: R¹, R² and R⁴ are hydrogen and R³ is hydrogenor chloro.
 6. The compound of claim 5 where: R⁵ is benzyl; U—V is—N(R⁶)—CH₂—CH₂—; and R⁶ is p-methyl-benzoyl.
 7. The compound of claim 1,selected from:3-benzyl-7-chloro-2-[1-(4-methyl-benzyl)-pyrrolidin-2-yl]-3H-quinazolin-4-one;3-benzyl-7-chloro-2-[1-(4-methyl-benzoyl)-pyrrolidin-2-yl]-3H-quinazolin-4-one;3-beznyl-7-chloro-2-[1-(4-methyl-benzoyl)-piperidin-2-yl]-3H-quinazolin-4-one;3-beznyl-7-chloro-2-[1-(4-methyl-benzyl)-piperidin-3-yl]-3H-quinazolin-4-one;3-beznyl-7-chloro-2-[1-(4-methyl-benzoyl)-piperidin-3-yl]-3H-quinazolin-4-one;3-benzyl-7-chloro-2-[1-(4-methyl-benzyl)-piperidin-4-yl]-3H-quinazolin-4-one;and3-benzyl-7-chloro-2-[1-(4-methyl-benzoyl)-piperidin-4-yl]-3H-quinazolin-4-one.8. The compound of claim 7 that is an (R)-enantiomer.
 9. The compound ofclaim 1, selected from:3-benzyl-7-chloro-2-[1-(4-methyl-benzoyl)-pyrrolidin-2-yl]-3H-quinazolin-4-one;3-benzyl-7-chloro-2-[1-(4-methyl-benzoyl)-piperidin-2-yl]-3H-quinazolin-4-one;3-benzyl-7-chloro-2-[1-(4-methyl-benzoyl)-piperidin-3-yl]-3H-quinazolin-4-one;and3-benzyl-7-chloro-2-[1-(4-methyl-benzoyl)-piperidin-4-yl]-3H-quinazolin-4-one.10. The compound of claim 9 that is an (R)-enantiomer.
 11. The compoundof claim 1, selected from:3-benzyl-7-chloro-2-[1-(4-methyl-benzoyl)-piperidin-2-yl]-3H-quinazolin-4-one; and3-benzyl-7-chloro-2-[1-(4-methyl-benzoyl)-piperidin-3-yl]-3H-quinazolin-4-one, especially the (R)-enantiomers thereof.12. The compound of claim 11 that is an (R)-enantiomer.
 13. Apharmaceutical formulation comprising a pharmaceutical acceptableexcipient and an effective amount of a compound of claim
 1. 14. A methodof treatment comprising administering an effective amount of a compoundof claim 1 to a patient suffering from a cellular proliferative disease.15. The method of claim 14 wherein the cellular proliferative disease iscancer, hyperplasia, restenosis, cardiac hypertrophy, an immune disorderor inflammation.
 16. A method of treatment for a cellular proliferativedisease comprising administering to a patient suffering therefrom acompound of claim 1 in an amount sufficient to modulate KSP kinesinactivity in cells affected with the disease.
 17. A kit comprising acompound of claim 1 and a package insert or other labeling includingdirections for treating a cellular proliferative disease byadministering an effective amount of said compound.
 18. A compound ofthe group represented by Formula II:

where: the dashed line indicates that the corresponding bond may be asingle bond or a double bond; T is a covalent bond or optionallysubstituted lower alkylene; U—V is chosen from —N(R⁶)—CR^(e)R^(f)—,—CR^(e)R^(f)—N(R⁶)—, —N(R⁶)—CR^(g)R^(h)—,—CR^(e)R^(f)—N(R⁶)—CR^(g)R^(h)—, and —CR^(e)R^(f)—CR^(g)R^(h)—N(R⁶)—; W,X and Y are independently —N═, N, —C═, CH, CR^(i), O or S; Z is —N═, N,—C═, CH, CR or is absent, provided that: no more than two of W, X, Y andZ are-N=, and W, X or Y can be O or S only when Z is absent; R^(i) isalkyl, alkoxy, halogen, cyano or substituted alkyl; R^(a), R^(b), R^(c),R^(d), R^(e), R^(f), R^(g) and R^(h) are independently chosen fromhydrogen, alkyl, aryl, aralkyl, heteroaryl, substituted alkyl,substituted aryl, substituted aralkyl and substituted heteroaryl; R¹,R², R³, and R⁴ are independently chosen from hydrogen, alkyl, alkoxy,halogen, cyano and substituted alkyl; R⁵ is alkyl, aryl, aralkyl,heteroaryl, heteroaralkyl, substituted alkyl, substituted aryl,substituted aralkyl, substituted heteroaryl or substitutedheteroaralkyl; and R⁶ is chosen from hydrogen, acyl, alkyl, aryl,aralkyl, heteroaryl, substituted acyl, substituted alkyl, substitutedaryl, substituted aralkyl and substituted heteroaryl; provided that R¹,R², R³ or R⁴ is absent where W, X, Y or Z, respectively, is —N═, O, S orabsent; or a pharmaceutical acceptable salt or solvate thereof.
 19. Thecompound of claim 18 comprising one or more of the following: T is acovalent bond, C₁ to C₄ alkylene or C₁ to C₄ alkylene substituted withhalo or oxo; W, X, Y and Z are independently —C═ or —N═; R¹, R², R³ andR⁴ are independently hydrogen, chloro, fluoro, methyl, methoxy, cyano orsubstituted lower alkyl; R⁵ is aralkyl or substituted aralkyl; R^(a) toR^(h) are independently hydrogen, lower alkyl or substituted loweralkyl; U—V is —N(R⁶)—CR^(e)R^(f)—CR^(g)R^(h),—CR^(e)R^(f)—N(R⁶)—CR^(g)R^(h) or —CR^(e)R^(f)—CR^(g)R^(h)—N(R⁶)—; R⁶ isoptionally substituted aralkyl or optionally substituted acyl; and is an(R)-enantiomer.
 20. The compound of claim 19 comprising one or more ofthe following: T is a covalent bond or C₁ to C₄ alkylene; R¹, R², R³ andR⁴ are independently hydrogen, chloro, fluoro, methyl, methoxy or cyano;R⁵ is benzyl or substituted benzyl; no more than one of R^(a) to R^(h)is other than hydrogen; U—V is —N(R⁶)—CR^(e)R^(f)—CR^(g)R^(h) or—CR^(e)R^(f)—N(R⁶)—CR^(g)R^(h)—; and R⁶ is optionally substituted acyl.21. The compound of claim 20 comprising one or more of the following: Tis a covalent bond; R¹, R², R³ and R⁴ are hydrogen, or three of R¹, R²,R³ and R⁴ are hydrogen and the fourth is halo, methoxy, methyl or cyano;R⁵ is benzyl; R^(a) to R^(h) are hydrogen; U—V is—N(R⁶)—CR^(e)R^(f)—C^(g)R^(h); and R⁶ is p-methyl-benzoyl.
 22. Thecompound of claim 21 where: T is a covalent bond; R¹, R² and R⁴ arehydrogen and R³ is hydrogen or chloro; R⁵ is benzyl; U—V is—N(R⁶)—CH₂—CH₂—; and R⁶ is p-methyl-benzoyl.
 23. A pharmaceuticalformulation comprising a pharmaceutically acceptable excipient and aneffective amount of a compound of claim
 18. 24. A method of treatmentcomprising administering an effective amount of a compound of claim 18to a patient suffering from a cellular proliferative disease.
 25. Themethod of claim 24 wherein the cellular proliferative disease is cancer,hyperplasia, restenosis, cardiac hypertrophy, an immune disorder orinflammation.
 26. A method of treatment for a cellular proliferativedisease comprising administering to a patient suffering therefrom acompound of claim 18 in an amount sufficient to modulate KSP kinesinactivity in cells affected with the disease.
 27. A kit comprising acompound of claim 18 and a package insert or other labeling includingdirections for treating a cellular proliferative disease byadministering an effective amount of said compound.